AB103418

Anti-RAB27B antibody

Name: Anti-RAB27B antibody (ab103418) | Abcam
Brand: Abcam Limited
SKU: AB103418
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal RAB27B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB27B.

View Alternative Names

Ras-related protein Rab-27B, C25KG, RAB27B

3 Images

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Western blot - Anti-RAB27B antibody (AB103418)

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Western blot - Anti-RAB27B antibody (ab103418) at 5 µg/mL

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HeLa cell lysate at 50 µg

Predicted band size: 25 kDa

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Western blot - Anti-RAB27B antibody (AB103418)

All lanes:

Western blot - Anti-RAB27B antibody (ab103418) at 5 µg/mL

Lane 1:

RAB27B transfected 293T cell lysate at 25 µg

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Non-transfected 293T cell lysate at 25 µg

Predicted band size: 25 kDa

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Western blot - Anti-RAB27B antibody (AB103418)

All lanes:

Western blot - Anti-RAB27B antibody (ab103418) at 5 µg/mL

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Human spleen tissue lysate at 50 µg

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAB27B.

O00194

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB27B also known as Ras-related protein Rab-27B belongs to the Rab family of small GTPases and has a molecular mass of approximately 25 kDa. RAB27B is involved in intracellular vesicle trafficking processes. It is expressed in various tissues with notable expression in platelets and secretory cells. The protein cycles between GDP-bound inactive and GTP-bound active states regulates cargo delivery and maintains distinct cellular compartments.

Biological function summary

The functional role of RAB27B includes mediating the transport and exocytosis of secretory vesicles. This protein collaborates with effector molecules such as MLPH (melanophilin) as part of a multiprotein complex that facilitates vesicle membrane docking and fusion. RAB27B's activity ensures proper cellular secretion processes impacting hormone release pigment distribution and other essential secretory pathways.

Pathways

RAB27B participates in the exosome secretion and regulated secretory pathways. It closely interacts with other proteins including RAB27A which shares similar trafficking functions. RAB27B plays a role in specialized secretory pathways affecting the maturation and docking of vesicles in cells like melanocytes and cytotoxic T lymphocytes which are essential for efficient immune responses and pigmentation respectively.

RAB27B is relevant in conditions such as cancer and Griscelli syndrome. Its dysregulation can contribute to tumor progression and metastasis due to altered vesicle trafficking and secretion dynamics. In Griscelli syndrome type 2 mutations in the related protein RAB27A although not directly in RAB27B impact melanosome transport leading to pigmentary issues and immune dysfunction. Understanding RAB27B's involvement offers insights into potential therapeutic strategies targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21775604, PubMed : 30771381). RAB27B regulates homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed : 30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed : 21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).

See full target information RAB27B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 20:30-39 PubMed36581340

2022

Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Nambara,Takaaki Masuda,Kosuke Hirose,Qingjiang Hu,Taro Tobo,Yuki Ozato,Junji Kurashige,Yoshiki Hiraki,Yuichi Hisamatsu,Tomohiro Iguchi,Keishi Sugimachi,Eiji Oki,Tomoharu Yoshizumi,Koshi Mimori

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Environmental toxicology 36:2174-2185 PubMed34291859

2021

MiR-186-5p suppresses cell migration, invasion, and epithelial mesenchymal transition in bladder cancer by targeting RAB27A/B.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjin Zhang,Lin Hao,Zhiyong Shen,Fengye Wang,Conghui Han

Oncology letters 16:1243-1252 PubMed29963197

2018

MicroRNA-599 suppresses glioma progression by targeting RAB27B.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jiang,Xiaohui Wang,Ji Zhang,Renchun Lai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB27B antibody

Western blot - Anti-RAB27B antibody (AB103418)

Western blot - Anti-RAB27B antibody (AB103418)

Western blot - Anti-RAB27B antibody (AB103418)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise