AB76779

Anti-RAB27B antibody

Name: Anti-RAB27B antibody (ab76779) | Abcam
Brand: Abcam Limited
SKU: AB76779
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(7 Publications)

Mouse Polyclonal RAB27B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB27B.

View Alternative Names

Ras-related protein Rab-27B, C25KG, RAB27B

1 Images

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Western blot - Anti-RAB27B antibody (AB76779)

All lanes:

Western blot - Anti-RAB27B antibody (ab76779) at 1/500 dilution

Lane 1:

RAB27B transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAB27B.

O00194

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB27B also known as Ras-related protein Rab-27B belongs to the Rab family of small GTPases and has a molecular mass of approximately 25 kDa. RAB27B is involved in intracellular vesicle trafficking processes. It is expressed in various tissues with notable expression in platelets and secretory cells. The protein cycles between GDP-bound inactive and GTP-bound active states regulates cargo delivery and maintains distinct cellular compartments.

Biological function summary

The functional role of RAB27B includes mediating the transport and exocytosis of secretory vesicles. This protein collaborates with effector molecules such as MLPH (melanophilin) as part of a multiprotein complex that facilitates vesicle membrane docking and fusion. RAB27B's activity ensures proper cellular secretion processes impacting hormone release pigment distribution and other essential secretory pathways.

Pathways

RAB27B participates in the exosome secretion and regulated secretory pathways. It closely interacts with other proteins including RAB27A which shares similar trafficking functions. RAB27B plays a role in specialized secretory pathways affecting the maturation and docking of vesicles in cells like melanocytes and cytotoxic T lymphocytes which are essential for efficient immune responses and pigmentation respectively.

RAB27B is relevant in conditions such as cancer and Griscelli syndrome. Its dysregulation can contribute to tumor progression and metastasis due to altered vesicle trafficking and secretion dynamics. In Griscelli syndrome type 2 mutations in the related protein RAB27A although not directly in RAB27B impact melanosome transport leading to pigmentary issues and immune dysfunction. Understanding RAB27B's involvement offers insights into potential therapeutic strategies targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed : 30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed : 21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).

See full target information RAB27B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed36077620

2022

Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Sik Park,Jong-In Kim,Chan-Hyeong Lee,Ju-Hyun Bae,Ju-Mi Park,Eun-Ji Choe,Moon-Chang Baek

Cell reports 39:110698 PubMed35443182

2022

Bladder epithelial cell phosphate transporter inhibition protects mice against uropathogenic Escherichia coli infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Pang,Zhihui Cheng,Si Zhang,Shujie Li,Xueping Li,Xiaodan Li,Xiao Zhang,Xiaoxiao Li,Yingxing Feng,Heting Cui,Zhen Chen,Le Liu,Qing Li,Jianxiao Huang,Mingqing Zhang,Siwei Zhu,Lei Wang,Lu Feng

Bioengineered 13:5103-5112 PubMed35164665

2022

RAB27B inhibits proliferation and promotes apoptosis of leukemic cells via 3-Hydroxy butyrate dehydrogenase 2 (BDH2).

Applications

Unspecified application

Species

Unspecified reactive species

Can Meng,Li Huang,Xiangjun Fu,Bin Wu,Lie Lin

Journal of experimental & clinical cancer research : CR 40:353 PubMed34753494

2021

Therapeutic inhibition of GAS6-AS1/YBX1/MYC axis suppresses cell propagation and disease progression of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Wei Liu,Yongming Zhou,Zhenya Hong,Jian Ni,Xiaoping Zhang,Ziping Li,Mengyuan Li,Wenjuan He,Donghua Zhang,Xuexing Chen,Jianhua Zhu

The Journal of biological chemistry 295:8005-8016 PubMed32350025

2020

The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Underwood,Bing Wang,Christine Carico,Robert H Whitaker,William J Placzek,Talene A Yacoubian

The Journal of general physiology 151:1386-1403 PubMed31676484

2019

Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique C Stephens,Nicole Osunsanmi,Kem A Sochacki,Tyrel W Powell,Justin W Taraska,Dinari A Harris

American journal of human genetics 91:1115-21 PubMed23176819

2012

Germline Mutation in EXPH5 Implicates the Rab27B Effector Protein Slac2-b in Inherited Skin Fragility.

Applications

Unspecified application

Species

Unspecified reactive species

John A McGrath,Kristina L Stone,Rumena Begum,Michael A Simpson,Patricia J Dopping-Hepenstal,Lu Liu,James R McMillan,Andrew P South,Celine Pourreyron,W H Irwin McLean,Anna E Martinez,Jemima E Mellerio,Maddy Parsons

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RAB27B antibody

Western blot - Anti-RAB27B antibody (AB76779)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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