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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal RAB29 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
The small GTPases Rab are key regulators in vesicle trafficking (PubMed:24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed:29212815). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed:22042847).
Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29, RAB29, RAB7L1
Rabbit Recombinant Monoclonal RAB29 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29, RAB29, RAB7L1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
MJF-R30-104
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody was developed with support from The Michael J. Fox Foundation.
RAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.
RAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.
RAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.
RAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
False colour image of Western blot: Anti-RAB29 antibody [MJF-R30-104] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab256527 was shown to bind specifically to RAB29. A band was observed at 23 kDa in wild-type A549 cell lysates with no signal observed at this size in RAB29 knockout cell line ab280040 (knockout cell lysate ab280099). To generate this image, wild-type and RAB29 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-104] (AB256527) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: RAB29 knockout A549 cell lysate at 20 µg
Lane 3: MCF-7 cell lysate at 20 µg
Lane 4: Caco-2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
This image was kindly provided by Dr. Mark Cookson, NIH
Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer
All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-104] (AB256527) at 1/4000 dilution
Lane 1: HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate 15ug
Lane 2: HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate 15ug
All lanes: IR Dye 800CW Goat anti-rabbit IgG (#926-32211) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256527 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256527 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab256527 IP in A549 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256527 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 30 seconds
All lanes: Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-104] (AB256527)
Predicted band size: 23 kDa
The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: 59 seconds
All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-104] (AB256527) at 1/1000 dilution
Lane 1: Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: 59 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256527).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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