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Rabbit Recombinant Monoclonal RAB29 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (AB256547), expandable thumbnail
  • Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (AB256547), expandable thumbnail
  • Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (AB256547), expandable thumbnail
  • Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (AB256547), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Not recommended
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

The small GTPases Rab are key regulators in vesicle trafficking (PubMed:24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed:29212815, PubMed:38127736). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed:22042847).

Alternative names

RAB7L1, RAB29, Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29

Recommended products

Rabbit Recombinant Monoclonal RAB29 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
MJF-R30-124
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab256547 is the carrier-free version of Anti-RAB29 antibody [MJF-R30-124] ab256526.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.

Biological function summary

RAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.

Pathways

RAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.

Associated diseases and disorders

RAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547), expandable thumbnail

    Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547)

    False colour image of Western blot: Anti-RAB29 antibody [MJF-R30-124] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-RAB29 antibody [MJF-R30-124] ab256526 was shown to bind specifically to RAB29. A band was observed at 23 kDa in wild-type A549 cell lysates with no signal observed at this size in RAB29 knockout cell line Human RAB29 knockout A549 cell line ab280040 (knockout cell lysate None). To generate this image, wild-type and RAB29 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-124] (Anti-RAB29 antibody [MJF-R30-124] ab256526) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: RAB29 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human RAB29 knockout A549 cell line (Human RAB29 knockout A549 cell line ab280040)

    Lane 2: Western blot - Human RAB29 knockout A549 cell lysate (Human RAB29 knockout A549 cell lysate ab280099)

    Lane 3: MCF-7 cell lysate at 20 µg

    Lane 4: Caco-2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547), expandable thumbnail

    Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547)

    This antibody reacts with unidentifiable proteins around 30,75 and 150 kDa. This image was kindly provided by Dr. Mark Cookson, NIH.

    Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer.

    Exposure Time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB29 antibody [MJF-R30-124] ab256526).

    All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-124] (Anti-RAB29 antibody [MJF-R30-124] ab256526) at 1/3000 dilution

    Lane 1: wildtype C57BL6 mouse lung tissue lysate at 15 µg

    Lane 2: Rab29 knock-out C57BL6 mouse lung tissue lysate at 15 µg

    Secondary

    All lanes: IR Dye 800CW Goat anti-rabbit IgG (#926-32211) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

  • Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547), expandable thumbnail

    Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547)

    RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with Anti-RAB29 antibody [MJF-R30-124] ab256526 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RAB29 antibody [MJF-R30-124] ab256526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: Anti-RAB29 antibody [MJF-R30-124] ab256526 IP in A549 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RAB29 antibody [MJF-R30-124] ab256526 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB29 antibody [MJF-R30-124] ab256526).

    All lanes: Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] (Anti-RAB29 antibody [MJF-R30-124] ab256526)

    Predicted band size: 23 kDa

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547), expandable thumbnail

    Western blot - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (ab256547)

    This data was developed using Anti-RAB29 antibody [MJF-R30-124] ab256526, the same antibody clone in a different buffer formulation.

    Western blot: Rabbit Monoclonal[MJF-R30-124] to RAB29 Anti-RAB29 antibody [MJF-R30-124] ab256526 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 23 kDa in Wild-type U-87 MG ab278079 cell lysates with no signal observed at this size in RAB29 knockout U-87 MG ab306765 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-RAB29 antibody [MJF-R30-124] (Anti-RAB29 antibody [MJF-R30-124] ab256526) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG ab278079 at 20 µg

    Lane 2: RAB29 knockout U-87 MG ab306765 at 20 µg

    Lane 3: MCF7 at 20 µg

    Lane 4: Caco-2 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

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Product protocols

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