JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241062

Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal RAB29 phospho T71 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 8 publications.

View Alternative Names

RAB7L1, RAB29, Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29

4 Images
Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)
  • WB

Supplier Data

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% BSA/TBST.

The blot was scanned with LI-COR Odyssey CLx.

This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

All lanes:

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB3 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 2:

HEK-293 overexpressing HA-tagged RAB5 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 3:

HEK-293 overexpressing HA-tagged RAB8 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 4:

HEK-293 overexpressing HA-tagged RAB10 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 5:

HEK-293 overexpressing HA-tagged RAB12 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 6:

HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 7:

HEK-293 overexpressing HA-tagged RAB35 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Lane 8:

HEK-293 overexpressing HA-tagged RAB43 and LRRK2 [Y1699C], whole cell lysate at 0.5 µg

Secondary

All lanes:

IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)
  • WB

Supplier Data

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% BSA/TBST.

The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins. While MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID : 26407721).

The blot was scanned with LI-COR Odyssey CLx.

This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee

All lanes:

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/500 dilution

Lane 1:

MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg

Lane 2:

HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg

Lane 3:

HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg

Lane 4:

HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg

Lane 5:

HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg

Secondary

All lanes:

IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)
  • WB

Lab

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney) over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

Lane 2:

MLi-2 (100nM for 60min) treated HEK-293 over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)
  • Dot

Unknown

Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.

Lane 1 : RAB29 (phospho T71) peptide.
Lane 2 : RAB29 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R24-17-1

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.
Biological function summary

RAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.

Pathways

RAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.

RAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The small GTPases Rab are key regulators in vesicle trafficking (PubMed : 24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed : 24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed : 29212815, PubMed : 38127736). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed : 38127736). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed : 22042847).
See full target information RAB29 phospho T71
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37874635

2023

Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson's disease-linked LRRK2 kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Herschel S Dhekne,Francesca Tonelli,Wondwossen M Yeshaw,Claire Y Chiang,Charles Limouse,Ebsy Jaimon,Elena Purlyte,Dario R Alessi,Suzanne R Pfeffer

The Biochemical journal 479:1759-1783 PubMed35950872

2022

Impact of 100 LRRK2 variants linked to Parkinson's disease on kinase activity and microtubule binding.

Applications

Unspecified application

Species

Unspecified reactive species

Alexia F Kalogeropulou,Elena Purlyte,Francesca Tonelli,Sven M Lange,Melanie Wightman,Alan R Prescott,Shalini Padmanabhan,Esther Sammler,Dario R Alessi

NPJ Parkinson's disease 8:92 PubMed35853899

2022

LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kedariti,Emanuele Frattini,Pascale Baden,Susanna Cogo,Laura Civiero,Elena Ziviani,Gianluca Zilio,Federico Bertoli,Massimo Aureli,Alice Kaganovich,Mark R Cookson,Leonidas Stefanis,Matthew Surface,Michela Deleidi,Alessio Di Fonzo,Roy N Alcalay,Hardy Rideout,Elisa Greggio,Nicoletta Plotegher

NPJ Parkinson's disease 8:73 PubMed35676398

2022

Distinct profiles of LRRK2 activation and Rab GTPase phosphorylation in clinical samples from different PD cohorts.

Applications

Unspecified application

Species

Unspecified reactive species

Lilian Petropoulou-Vathi,Athina Simitsi,Politymi-Eleni Valkimadi,Maria Kedariti,Lampros Dimitrakopoulos,Christos Koros,Dimitra Papadimitriou,Alexandros Papadimitriou,Leonidas Stefanis,Roy N Alcalay,Hardy J Rideout

The Journal of cell biology 221: PubMed35266954

2022

The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne E D Stormo,Farbod Shavarebi,Molly FitzGibbon,Elizabeth M Earley,Hannah Ahrendt,Lotus S Lum,Erik Verschueren,Danielle L Swaney,Gaia Skibinski,Abinaya Ravisankar,Jeffrey van Haren,Emily J Davis,Jeffrey R Johnson,John Von Dollen,Carson Balen,Jacob Porath,Claudia Crosio,Christian Mirescu,Ciro Iaccarino,William T Dauer,R Jeremy Nichols,Torsten Wittmann,Timothy C Cox,Steve Finkbeiner,Nevan J Krogan,Scott A Oakes,Annie Hiniker

The Biochemical journal 478:3555-3573 PubMed34515301

2021

Impact of Type II LRRK2 inhibitors on signaling and mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tasegian,Francois Singh,Ian G Ganley,Alastair D Reith,Dario R Alessi

Current biology : CB 31:2140-2154.e6 PubMed33765413

2021

Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

C Alexander Boecker,Juliet Goldsmith,Dan Dou,Gregory G Cajka,Erika L F Holzbaur

The Journal of cell biology 218:4157-4170 PubMed31624137

2019

Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel C Gomez,Paulina Wawro,Pawel Lis,Dario R Alessi,Suzanne R Pfeffer
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB256527

Anti-RAB29 antibody [MJF-R30-104]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-104] (AB256527)

  • 0
  • 0
Primary Antibodies

AB256547

Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] - BSA and Azide free (AB256547)

  • 0
  • 0
Primary Antibodies

AB256548

Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (AB256548)

  • 0
  • 0
Primary Antibodies

AB256526

Anti-RAB29 antibody [MJF-R30-124]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] (AB256526)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB230261

Anti-RAB10 (phospho T73) antibody [MJF-R21]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

  • 0
  • 0
Primary Antibodies

AB203181

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (AB203181)

  • 0
  • 0
Primary Antibodies

AB256487

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

  • 0
  • 0
Primary Antibodies

AB230260

Anti-RAB8A (phospho T72) antibody [MJF-R20]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] (AB230260)

  • 4
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com