Rabbit Recombinant Monoclonal RAB29 phospho T71 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Images
![Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (AB243289), expandable thumbnail](https://content.abcam.com/products/images/rab29-phospho-t71-antibody-mjf-r24-17-1-bsa-and-azide-free-ab243289--western-blot-img142732.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (AB243289)")
![Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (AB243289), expandable thumbnail](https://content.abcam.com/products/images/rab29-phospho-t71-antibody-mjf-r24-17-1-bsa-and-azide-free-ab243289--dot-blot-img8464.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (AB243289)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Expected | Tested |
| Synthetic peptide | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
The small GTPases Rab are key regulators in vesicle trafficking (PubMed:24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed:29212815, PubMed:38127736). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed:22042847).
Alternative names
RAB7L1, RAB29, Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29
Recommended products
Rabbit Recombinant Monoclonal RAB29 phospho T71 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- MJF-R24-17-1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243289 is the carrier-free version of Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.
- Biological function summary
RAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.
- Pathways
RAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.
- Associated diseases and disorders
RAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289), expandable thumbnail](https://content.abcam.com/products/images/rab29-phospho-t71-antibody-mjf-r24-17-1-bsa-and-azide-free-ab243289--western-blot-img142732.jpg "Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)")
Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062).
All lanes: Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062) at 1/500 dilution
Lane 1: MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 2: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 3: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
Lane 4: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
Lane 5: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg
SecondaryAll lanes: IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
![Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289), expandable thumbnail](https://content.abcam.com/products/images/rab29-phospho-t71-antibody-mjf-r24-17-1-bsa-and-azide-free-ab243289--dot-blot-img8464.jpg "Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)")
Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
Dot blot analysis of RAB29 (phospho T71) labeled with Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062 at 1/1000 dilution.
Lane 1: RAB29 (phospho T71) peptide.
Lane 2: RAB29 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062).
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