AB230881

Anti-RAB31 antibody

Name: Anti-RAB31 antibody (ab230881) | Abcam
Brand: Abcam Limited
SKU: AB230881

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(4 Publications)

Rabbit Polyclonal RAB31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RAB31 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RAB22B, RAB31, Ras-related protein Rab-31, Ras-related protein Rab-22B

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody (AB230881)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody (AB230881)

Formalin-fixed, paraffin-embedded human colon, MALT and vessels stained for RAB31 using ab230881 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RAB31 antibody (AB230881)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB31 antibody (AB230881)

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for RAB31 using ab230881 at 1/100 dilution in ICC/IF.

Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight^® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Supplier Data

Western blot - Anti-RAB31 antibody (AB230881)

All lanes:

Western blot - Anti-RAB31 antibody (ab230881) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lane 3:

Rat brain lysate

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human RAB31 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13636

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB31 also known as RAB22B belongs to the RAB family which plays an important role in vesicular trafficking. It is a small GTPase with a mass of approximately 23 kDa. RAB31 is mainly expressed in epithelial tissues and certain cancer cell lines. It regulates vesicle formation movement and fusion by cycling between an active GTP-bound state and an inactive GDP-bound state. This cycling is important for its function in the intracellular transport system.

Biological function summary

RAB31 influences early endosome dynamics and directly impacts the sorting and recycling of receptors. It associates with effector proteins to form protein complexes that facilitate endocytosis and exocytosis processes. It also interacts with other Rab proteins to ensure proper membrane specificity and temporal regulation within cellular compartments. This behavior highlights its role in maintaining cellular homeostasis and responsiveness to environmental changes.

Pathways

Studies have shown that RAB31 contributes significantly to the endocytic pathway and the insulin signaling pathway. It has functional interactions with proteins like RAB5 and RAB11 both of which are key regulators of endosomal trafficking. RAB31 ensures a proper transport cycle that influences receptor recycling and signaling impacting how cells respond to growth factors and hormones.

Research implicates RAB31 in the progression of certain cancers such as breast cancer due to its established role in vesicle trafficking and receptor recycling which can affect cell growth and division. Its altered expression levels may intersect with proteins such as EGFR leading to abnormal signaling cascades. Furthermore RAB31 has associations with metabolic disorders as disruptions in insulin signaling pathways may contribute to diabetes showcasing its diverse influence on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 11784320). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

See full target information RAB31

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 5:32 PubMed39138733

2024

Effects of HOX family regulator-mediated modification patterns and immunity characteristics on tumor-associated cell type in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

JiaoLin Yang,JinPeng Li,SuFen Li,YuTong Yang,HuanCheng Su,HongRui Guo,Jing Lei,YaLin Wang,KaiTing Wen,Xia Li,SanYuan Zhang,Zhe Wang

Journal of cellular and molecular medicine 26:3153-3168 PubMed35322540

2022

LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Kang Ho,Danning Zheng,Jiaming Sun,Dongsheng Wen,Shan Wu,Li Yu,Ya Gao,Yifan Zhang,Qingfeng Li

Oncology reports : PubMed33469675

2021

High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoguang Li,Fengshuo Zhu,Zhonglong Liu,Xiao Tang,Yu Han,Junjian Jiang,Chunyue Ma,Yue He

Oncology letters 20:244 PubMed32973957

2020

RAB31 is targeted by miR-26b and serves a role in the promotion of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wu,Qiong Feng,Yanfei Xiong,Xing Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB31 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody (AB230881)

Immunocytochemistry/ Immunofluorescence - Anti-RAB31 antibody (AB230881)

Western blot - Anti-RAB31 antibody (AB230881)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise