AB224421

Anti-RAB31 antibody - C-terminal

Name: Anti-RAB31 antibody - C-terminal (ab224421) | Abcam
Brand: Abcam Limited
SKU: AB224421
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RAB31 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RAB31 aa 100 to C-terminus.

View Alternative Names

RAB22B, RAB31, Ras-related protein Rab-31, Ras-related protein Rab-22B

3 Images

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Western blot - Anti-RAB31 antibody - C-terminal (AB224421)

All lanes:

Western blot - Anti-RAB31 antibody - C-terminal (ab224421) at 0.4 µg/mL

Lane 1:

Human HeLa cell line

Lane 2:

Human A-431 cell line

Predicted band size: 22 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody - C-terminal (AB224421)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody - C-terminal (AB224421)

Paraffin-embedded human adrenal gland tissue stained for RAB31 using ab224421 at 1/20 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RAB31 antibody - C-terminal (AB224421)

All lanes:

Western blot - Anti-RAB31 antibody - C-terminal (ab224421) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RAB31 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13636

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20.00000 - 1/50.00000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB31 also known as RAB22B belongs to the RAB family which plays an important role in vesicular trafficking. It is a small GTPase with a mass of approximately 23 kDa. RAB31 is mainly expressed in epithelial tissues and certain cancer cell lines. It regulates vesicle formation movement and fusion by cycling between an active GTP-bound state and an inactive GDP-bound state. This cycling is important for its function in the intracellular transport system.

Biological function summary

RAB31 influences early endosome dynamics and directly impacts the sorting and recycling of receptors. It associates with effector proteins to form protein complexes that facilitate endocytosis and exocytosis processes. It also interacts with other Rab proteins to ensure proper membrane specificity and temporal regulation within cellular compartments. This behavior highlights its role in maintaining cellular homeostasis and responsiveness to environmental changes.

Pathways

Studies have shown that RAB31 contributes significantly to the endocytic pathway and the insulin signaling pathway. It has functional interactions with proteins like RAB5 and RAB11 both of which are key regulators of endosomal trafficking. RAB31 ensures a proper transport cycle that influences receptor recycling and signaling impacting how cells respond to growth factors and hormones.

Research implicates RAB31 in the progression of certain cancers such as breast cancer due to its established role in vesicle trafficking and receptor recycling which can affect cell growth and division. Its altered expression levels may intersect with proteins such as EGFR leading to abnormal signaling cascades. Furthermore RAB31 has associations with metabolic disorders as disruptions in insulin signaling pathways may contribute to diabetes showcasing its diverse influence on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

See full target information RAB31

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 159:1019-1035.e22 PubMed32446697

2020

Protein Kinase D1, Reduced in Human Pancreatic Tumors, Increases Secretion of Small Extracellular Vesicles From Cancer Cells That Promote Metastasis to Lung in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Milena Armacki,Sandra Polaschek,Mareike Waldenmaier,Mareen Morawe,Claudia Ruhland,Rebecca Schmid,André Lechel,Umesh Tharehalli,Christoph Steup,Yasin Bektas,Hongxia Li,Johann M Kraus,Hans A Kestler,Stephan Kruger,Steffen Ormanns,Paul Walther,Tim Eiseler,Thomas Seufferlein

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

Select an associated product type

Alternative Product

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