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AB103160

Anti-RAB32 antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal RAB32 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-32.

View Alternative Names

Ras-related protein Rab-32, RAB32

2 Images
Western blot - Anti-RAB32 antibody (AB103160)
  • WB

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Western blot - Anti-RAB32 antibody (AB103160)

All lanes:

Western blot - Anti-RAB32 antibody (ab103160) at 1 µg/mL

All lanes:

Human liver lysate at 50 µg

Predicted band size: 25 kDa

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Western blot - Anti-RAB32 antibody (AB103160)
  • WB

Unknown

Western blot - Anti-RAB32 antibody (AB103160)

All lanes:

Western blot - Anti-RAB32 antibody (ab103160) at 1 µg/mL

Lane 1:

RAB32 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Ras-related protein Rab-32.

Q13637

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB32 is a member of the RAB protein family involved in intracellular membrane trafficking. It is sometimes called Ras-related protein RAB32. The molecular mass of RAB32 is about 26 kilodaltons. It locates itself primarily in the cytoplasm and various organelles such as the mitochondria and endosomes. RAB32 has a role in vesicle trafficking acting as a molecular switch that toggles between active GTP-bound and inactive GDP-bound states managing the transport and fusion of cellular vesicles.
Biological function summary

The normal function of RAB32 influences several cellular processes including pigmentation and energy metabolism. It does not usually act alone but is part of larger protein complexes that regulate intracellular processes. RAB32 contributes to mitochondrial fission and fusion as well as melanosome biogenesis. Its interaction with other proteins helps establish a cellular environment compatible with various organelle functions.

Pathways

Its role is significant in key cellular pathways like the cAMP signaling pathway and the autophagy-lysosome pathway. RAB32 interacts with proteins such as PKA and ATG16L1 to modulate these pathways. In the cAMP pathway RAB32 affects protein kinase A activity modifying cellular responses to external signals. In the autophagy process it assists in lysosome function contributing to cellular cleanup and energy homeostasis.

The dysfunction of RAB32 has connections with some types of cancer and neurodegenerative diseases. Its overexpression or aberrant regulation is often seen in melanoma development where it can disrupt normal pigmentation processes. Additionally in neurodegenerative conditions like Alzheimer's disease RAB32 dysregulation might influence mitochondrial dynamics as it interacts with proteins like DRP1. Understanding these connections provides potential targets for therapeutic interventions in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 11784320, PubMed : 21808068). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 11784320). Also acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed : 12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed : 21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed : 23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed : 38127736).
See full target information Ras-related protein Rab-32
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 629:426-434 PubMed38658764

2024

PGE inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Morotti,Alizee J Grimm,Helen Carrasco Hope,Marion Arnaud,Mathieu Desbuisson,Nicolas Rayroux,David Barras,Maria Masid,Baptiste Murgues,Bovannak S Chap,Marco Ongaro,Ioanna A Rota,Catherine Ronet,Aspram Minasyan,Johanna Chiffelle,Sebastian B Lacher,Sara Bobisse,Clément Murgues,Eleonora Ghisoni,Khaoula Ouchen,Ribal Bou Mjahed,Fabrizio Benedetti,Naoill Abdellaoui,Riccardo Turrini,Philippe O Gannon,Khalil Zaman,Patrice Mathevet,Loic Lelievre,Isaac Crespo,Marcus Conrad,Gregory Verdeil,Lana E Kandalaft,Julien Dagher,Jesus Corria-Osorio,Marie-Agnes Doucey,Ping-Chih Ho,Alexandre Harari,Nicola Vannini,Jan P Böttcher,Denarda Dangaj Laniti,George Coukos

FEBS letters 593:2612-2627 PubMed31271652

2019

iTRAQ-based proteomic and bioinformatic characterization of human mast cells upon infection by the influenza A virus strains H1N1 and H5N1.

Applications

Unspecified application

Species

Unspecified reactive species

Hongping Wu,Shouping Zhang,Caiyun Huo,Shumei Zou,Zhengxing Lian,Yanxin Hu
View all publications
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Product promise

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