Rabbit Polyclonal RAB32 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus.
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Publications
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- Molecular therapy. Nucleic acids 26:603-6122021Reduced expression of miR-30c-5p promotes hepatocellular carcinoma progression by targeting RAB32.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng He,Meng Tian,Xuan FuPubMed 34703646
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13637
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736).
Alternative names
Ras-related protein Rab-32, RAB32
Recommended products
Rabbit Polyclonal RAB32 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13637
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB32 is a member of the RAB protein family involved in intracellular membrane trafficking. It is sometimes called Ras-related protein RAB32. The molecular mass of RAB32 is about 26 kilodaltons. It locates itself primarily in the cytoplasm and various organelles such as the mitochondria and endosomes. RAB32 has a role in vesicle trafficking acting as a molecular switch that toggles between active GTP-bound and inactive GDP-bound states managing the transport and fusion of cellular vesicles.
- Biological function summary
The normal function of RAB32 influences several cellular processes including pigmentation and energy metabolism. It does not usually act alone but is part of larger protein complexes that regulate intracellular processes. RAB32 contributes to mitochondrial fission and fusion as well as melanosome biogenesis. Its interaction with other proteins helps establish a cellular environment compatible with various organelle functions.
- Pathways
Its role is significant in key cellular pathways like the cAMP signaling pathway and the autophagy-lysosome pathway. RAB32 interacts with proteins such as PKA and ATG16L1 to modulate these pathways. In the cAMP pathway RAB32 affects protein kinase A activity modifying cellular responses to external signals. In the autophagy process it assists in lysosome function contributing to cellular cleanup and energy homeostasis.
- Associated diseases and disorders
The dysfunction of RAB32 has connections with some types of cancer and neurodegenerative diseases. Its overexpression or aberrant regulation is often seen in melanoma development where it can disrupt normal pigmentation processes. Additionally in neurodegenerative conditions like Alzheimer's disease RAB32 dysregulation might influence mitochondrial dynamics as it interacts with proteins like DRP1. Understanding these connections provides potential targets for therapeutic interventions in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB32 antibody (ab251764)
Paraffin-embedded human adrenal gland tissue stained for RAB32 using ab251764 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-RAB32 antibody (ab251764)
All lanes: Western blot - Anti-RAB32 antibody (ab251764) at 0.4 µg/mL
Lane 1: Sk-MEL-30 (Human cell line) cell lysate
Lane 2: MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 25 kDa
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