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AB251764

Anti-RAB32 antibody

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(3 Publications)

Rabbit Polyclonal RAB32 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus.

View Alternative Names

Ras-related protein Rab-32, RAB32

2 Images
Western blot - Anti-RAB32 antibody (AB251764)
  • WB

Unknown

Western blot - Anti-RAB32 antibody (AB251764)

All lanes:

Western blot - Anti-RAB32 antibody (ab251764) at 0.4 µg/mL

Lane 1:

Sk-MEL-30 (Human cell line) cell lysate

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) cell lysate

Predicted band size: 25 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB32 antibody (AB251764)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB32 antibody (AB251764)

Paraffin-embedded human adrenal gland tissue stained for RAB32 using ab251764 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Ras-related protein Rab-32 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13637

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB32 is a member of the RAB protein family involved in intracellular membrane trafficking. It is sometimes called Ras-related protein RAB32. The molecular mass of RAB32 is about 26 kilodaltons. It locates itself primarily in the cytoplasm and various organelles such as the mitochondria and endosomes. RAB32 has a role in vesicle trafficking acting as a molecular switch that toggles between active GTP-bound and inactive GDP-bound states managing the transport and fusion of cellular vesicles.
Biological function summary

The normal function of RAB32 influences several cellular processes including pigmentation and energy metabolism. It does not usually act alone but is part of larger protein complexes that regulate intracellular processes. RAB32 contributes to mitochondrial fission and fusion as well as melanosome biogenesis. Its interaction with other proteins helps establish a cellular environment compatible with various organelle functions.

Pathways

Its role is significant in key cellular pathways like the cAMP signaling pathway and the autophagy-lysosome pathway. RAB32 interacts with proteins such as PKA and ATG16L1 to modulate these pathways. In the cAMP pathway RAB32 affects protein kinase A activity modifying cellular responses to external signals. In the autophagy process it assists in lysosome function contributing to cellular cleanup and energy homeostasis.

The dysfunction of RAB32 has connections with some types of cancer and neurodegenerative diseases. Its overexpression or aberrant regulation is often seen in melanoma development where it can disrupt normal pigmentation processes. Additionally in neurodegenerative conditions like Alzheimer's disease RAB32 dysregulation might influence mitochondrial dynamics as it interacts with proteins like DRP1. Understanding these connections provides potential targets for therapeutic interventions in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed : 12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed : 21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed : 23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed : 38127736).
See full target information Ras-related protein Rab-32
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of international medical research 52:3000605241282384 PubMed39628429

2024

RAB32 promotes glioma cell progression by activating the JAK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sinan Zhang,Xudong Jiang,Qing Wei,Liji Huang,Zhuoyan Huang,Lina Zhang

The EMBO journal 42:e112845 PubMed37272163

2023

Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.

Applications

Unspecified application

Species

Unspecified reactive species

Ruheena Javed,Ashish Jain,Thabata Duque,Emily Hendrix,Masroor Ahmad Paddar,Sajjad Khan,Aurore Claude-Taupin,Jingyue Jia,Lee Allers,Fulong Wang,Michal Mudd,Graham Timmins,Keith Lidke,Tor Erik Rusten,Prithvi Reddy Akepati,Yi He,Fulvio Reggiori,Eeva-Liisa Eskelinen,Vojo Deretic

Molecular therapy. Nucleic acids 26:603-612 PubMed34703646

2021

Reduced expression of miR-30c-5p promotes hepatocellular carcinoma progression by targeting RAB32.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng He,Meng Tian,Xuan Fu
View all publications
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