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AB155679

Anti-RAB33B antibody

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(1 Publication)

Rabbit Polyclonal RAB33B antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Ras-related protein Rab-33B aa 1 to C-terminus.

View Alternative Names

Ras-related protein Rab-33B, RAB33B

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB33B antibody (AB155679)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB33B antibody (AB155679)

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling RAB33B with ab155679 antibody at 1/500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB33B antibody (AB155679)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB33B antibody (AB155679)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for RAB33B protein at cytosol with ab155679 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-RAB33B antibody (AB155679)
  • WB

Supplier Data

Western blot - Anti-RAB33B antibody (AB155679)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RAB33B antibody (ab155679) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extracts at 30 µg

Lane 2:

MDA-MB-231 whole cell extracts at 30 µg

Predicted band size: 26 kDa

false

Western blot - Anti-RAB33B antibody (AB155679)
  • WB

Unknown

Western blot - Anti-RAB33B antibody (AB155679)

12% SDS PAGE

All lanes:

Western blot - Anti-RAB33B antibody (ab155679) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Ras-related protein Rab-33B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H082

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB33B also known as Ras-related protein Rab-33B is a small GTPase involved in intracellular vesicle trafficking. It has a molecular mass of about 25 kDa. RAB33B localizes mainly to the Golgi apparatus. It functions in regulating vesicle movement within the Golgi and between the Golgi and other organelles. This protein is widely expressed in various tissues suggesting its broad role in cellular processes.
Biological function summary

RAB33B interacts actively with other proteins like Golgins and SNAREs to mediate vesicle budding transport and fusion. It is part of a complex that influences Golgi structure and function playing a role in maintaining cellular homeostasis. Through its GTPase activity RAB33B helps manage the directionality and specificity of vesicle trafficking. This positions it as an important player in cellular logistics ensuring protein and lipid cargoes reach their destination effectively.

Pathways

The protein's function links it to the post-Golgi vesicular transport pathway. This pathway is critical for transporting proteins from the Golgi to their final destinations including the cell membrane. RAB33B also integrates with the GTPase cycle pathway working alongside other proteins like RAB1 and RAB6 which facilitate different stages of vesicle transport. These interactions ensure the smooth progression of Golgi-related pathways affecting various cellular functions.

RAB33B has been associated with Smith-McCort dysplasia a bone development disorder. Mutations affecting RAB33B disrupt normal Golgi function leading to defects in collagen secretion which impacts skeletal formation. Additionally its interactions with proteins like RAB1 in the context of disease have drawn attention to its potential role in viral infections as RAB proteins often play roles in the life cycle of certain viruses. Understanding these connections can provide insights into therapeutic targets and strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 20163571, PubMed : 21808068). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 18448665, PubMed : 20163571, PubMed : 21808068). RAB33B acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (PubMed : 20163571). Participates in autophagosome formation by recruiting the ATG12-ATG5-ATG16L1 complex to phagophores, probably in a nucleotide-independent manner (PubMed : 18448665, PubMed : 32960676).
See full target information Ras-related protein Rab-33B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34523692

2021

Transcription factor HOXC10 activates the expression of MTFR2 to regulate the proliferation, invasion and migration of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xie,Ran Chen,Liujia Yan,Zhangjun Jia,Guangshu Liang,Qin Wang
View all publications
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Product promise

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