Rabbit Polyclonal PON1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB35 aa 1 to C-terminus.
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Publications
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- Aging 13:21435-214502021MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongqing Wen,Zhiyan Liu,Jingjing Tang,Lina BuPubMed 34500436
- Cell reports 26:3380-3390.e52019Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Faris,Marlena Merling,Shelby E Andersen,Cheryl A Dooley,Ted Hackstadt,Mary M WeberPubMed 30893609
- Nature communications 9:14752018A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSalvatore Corallino et. al.PubMed 29662076
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAB35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15286
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Alternative names
Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAY, RAB1C, RAB35
Recommended products
Rabbit Polyclonal PON1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB35 aa 1 to C-terminus.
Alternative names
Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAY, RAB1C, RAB35
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAB35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15286
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB35 is a small GTPase belonging to the Ras superfamily and is sometimes referred to as 'Rab GTP-binding protein' and 'RAB35 member RAS oncogene family'. The protein plays an important mechanical role in endocytic recycling and intracellular trafficking. It acts by cycling between an active GTP-bound and an inactive GDP-bound state which allows it to coordinate intracellular transport processes. RAB35 is a 23 kDa protein and is expressed in various tissues with high levels observed in the brain and immune cells.
- Biological function summary
RAB35 is necessary for maintaining endosomal sorting and recycling by facilitating the formation of tubular recycling compartments. It functions as part of a complex with other molecules like the exocyst complex which is needed for exocytosis. Through these interactions RAB35 ensures the proper distribution of proteins and lipids at the plasma membrane supporting cellular activities such as cell migration and synaptic function in neurons.
- Pathways
RAB35 has significant roles in both the endocytic and exocytic pathways operating alongside proteins such as ARF6 and EPIST. In the endocytic pathway RAB35 is involved in the recycling of endosomes ensuring that receptors and other proteins get recycled back to the cell surface. In the exocytic pathway it aids the exocyst complex which is important for vesicle tethering and fusion at the plasma membrane a process vital for cellular secretion and communication.
- Associated diseases and disorders
RAB35 relates to conditions such as cancer and neurological disorders. Dysregulation of RAB35 has been observed in several types of cancer where it may influence cell proliferation and invasion. RAB35 is also implicated in neurological disorders possibly due to its role in maintaining synaptic function and neuronal communication. In these contexts it has potential interactions with proteins like RAS oncogene family members and other molecules involved in cell growth and differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-RAB35 antibody (ab152138)
12% SDS-PAGE
All lanes: Western blot - Anti-RAB35 antibody (ab152138) at 1/3000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg
Lane 3: IMR32 (human brain neuroblast cell line) whole cell lysate at 30 µg
Lane 4: SK-N-AS (human brain neuroblastoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
Western blot - Anti-RAB35 antibody (ab152138)
False colour image of Western blot: Anti-RAB35 antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab152138 was shown to bind specifically to RAB35. A band was observed at 25 kDa in wild-type HeLa cell lysates with no signal observed at this size in RAB35 knockout cell line Human RAB35 knockout HeLa cell line ab265984 (knockout cell lysate Human RAB35 knockout HeLa cell lysate ab259076). To generate this image, wild-type and RAB35 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-RAB35 antibody (ab152138) at 1/500 dilution
Lanes 1 - 4: Western blot - Anti-RAB35 antibody (Anti-RAB35 antibody ab230838) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RAB35 knockout HeLa cell lysate at 20 µg
Lane 3: Caco-2 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa, 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB35 antibody (ab152138)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RAB35 with ab152138 at 1/250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/ Immunofluorescence - Anti-RAB35 antibody (ab152138)
Immunofluorescence analysis of 4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell labeling RAB35 with ab152138 at 1/500 dilution. Blue: Hoechst 33342 staining.
Western blot - Anti-RAB35 antibody (ab152138)
12% SDS-PAGE
All lanes: Western blot - Anti-RAB35 antibody (ab152138) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
Western blot - Anti-RAB35 antibody (ab152138)
12% SDS-PAGE
All lanes: Western blot - Anti-RAB35 antibody (ab152138) at 1/2000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
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