Rabbit Monoclonal PON1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.
Images
![Sandwich ELISA - Anti-RAB35 antibody [EPR24487-20] - BSA and Azide free (Detector) (AB307243), expandable thumbnail](https://content.abcam.com/products/images/rab35-antibody-epr24487-20-bsa-and-azide-free-detector-ab307243--sandwich-elisa-img403970.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-RAB35 antibody [EPR24487-20] - BSA and Azide free (Detector) (AB307243)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
| Mouse | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Alternative names
RAB1C, RAY, RAB35, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C
Recommended products
Rabbit Monoclonal PON1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24487-20
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB35 is a small GTPase belonging to the Ras superfamily and is sometimes referred to as 'Rab GTP-binding protein' and 'RAB35 member RAS oncogene family'. The protein plays an important mechanical role in endocytic recycling and intracellular trafficking. It acts by cycling between an active GTP-bound and an inactive GDP-bound state which allows it to coordinate intracellular transport processes. RAB35 is a 23 kDa protein and is expressed in various tissues with high levels observed in the brain and immune cells.
- Biological function summary
RAB35 is necessary for maintaining endosomal sorting and recycling by facilitating the formation of tubular recycling compartments. It functions as part of a complex with other molecules like the exocyst complex which is needed for exocytosis. Through these interactions RAB35 ensures the proper distribution of proteins and lipids at the plasma membrane supporting cellular activities such as cell migration and synaptic function in neurons.
- Pathways
RAB35 has significant roles in both the endocytic and exocytic pathways operating alongside proteins such as ARF6 and EPIST. In the endocytic pathway RAB35 is involved in the recycling of endosomes ensuring that receptors and other proteins get recycled back to the cell surface. In the exocytic pathway it aids the exocyst complex which is important for vesicle tethering and fusion at the plasma membrane a process vital for cellular secretion and communication.
- Associated diseases and disorders
RAB35 relates to conditions such as cancer and neurological disorders. Dysregulation of RAB35 has been observed in several types of cancer where it may influence cell proliferation and invasion. RAB35 is also implicated in neurological disorders possibly due to its role in maintaining synaptic function and neuronal communication. In these contexts it has potential interactions with proteins like RAS oncogene family members and other molecules involved in cell growth and differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-RAB35 antibody [EPR24487-20] - BSA and Azide free (Detector) (ab307243), expandable thumbnail](https://content.abcam.com/products/images/rab35-antibody-epr24487-20-bsa-and-azide-free-detector-ab307243--sandwich-elisa-img403970.jpg "Sandwich ELISA - Anti-RAB35 antibody [EPR24487-20] - BSA and Azide free (Detector) (ab307243)")
Sandwich ELISA - Anti-RAB35 antibody [EPR24487-20] - BSA and Azide free (Detector) (ab307243)
Sandwich ELISA of Human/Mouse RAB35 Antibody Pair - BSA and Azide free ab307241 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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