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Rabbit Recombinant Monoclonal PON1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-RAB35 antibody [MJF-R34-25] (AB288567), expandable thumbnail
  • Western blot - Anti-RAB35 antibody [MJF-R34-25] (AB288567), expandable thumbnail
  • Western blot - Anti-RAB35 antibody [MJF-R34-25] (AB288567), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Tested
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.

Alternative names

RAB1C, RAY, RAB35, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C

Recommended products

Rabbit Recombinant Monoclonal PON1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJF-R34-25
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB35 is a small GTPase belonging to the Ras superfamily and is sometimes referred to as 'Rab GTP-binding protein' and 'RAB35 member RAS oncogene family'. The protein plays an important mechanical role in endocytic recycling and intracellular trafficking. It acts by cycling between an active GTP-bound and an inactive GDP-bound state which allows it to coordinate intracellular transport processes. RAB35 is a 23 kDa protein and is expressed in various tissues with high levels observed in the brain and immune cells.

Biological function summary

RAB35 is necessary for maintaining endosomal sorting and recycling by facilitating the formation of tubular recycling compartments. It functions as part of a complex with other molecules like the exocyst complex which is needed for exocytosis. Through these interactions RAB35 ensures the proper distribution of proteins and lipids at the plasma membrane supporting cellular activities such as cell migration and synaptic function in neurons.

Pathways

RAB35 has significant roles in both the endocytic and exocytic pathways operating alongside proteins such as ARF6 and EPIST. In the endocytic pathway RAB35 is involved in the recycling of endosomes ensuring that receptors and other proteins get recycled back to the cell surface. In the exocytic pathway it aids the exocyst complex which is important for vesicle tethering and fusion at the plasma membrane a process vital for cellular secretion and communication.

Associated diseases and disorders

RAB35 relates to conditions such as cancer and neurological disorders. Dysregulation of RAB35 has been observed in several types of cancer where it may influence cell proliferation and invasion. RAB35 is also implicated in neurological disorders possibly due to its role in maintaining synaptic function and neuronal communication. In these contexts it has potential interactions with proteins like RAS oncogene family members and other molecules involved in cell growth and differentiation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567), expandable thumbnail

    Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    Lysates/proteins at 20 μg per lane.

    Performed under reducing conditions.

    False colour image of Western blot: Anti-RAB35 antibody [MJF-34-R25] (ab288567) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, ab288567 was shown to bind specifically to RAB35. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in RAB35 knockout cell lysates. To generate this image, wild-type and RAB35 knockout A549 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution.

    Wild-type A549 and RAB35 knockout A549 lysates were kindly provided by Prof. Dario Alessi University of Dundee.

    All lanes: Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567) at 1/1000 dilution

    Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: RAB35 knockout A549 whole cell lysate at 20 µg

    Lane 3: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 25 kDa

  • Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567), expandable thumbnail

    Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa

    Observed band size: 25 kDa

    Exposure time: 37s

  • Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567), expandable thumbnail

    Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody has no cross-reactivity with other RAB family proteins

    All lanes: Western blot - Anti-RAB35 antibody [MJF-R34-25] (ab288567) at 1/1000 dilution

    Lane 1: RAB35 full-length protein 10 ng

    Lane 2: RAB family protein homologous region 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa

    Observed band size: 25 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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