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AB249211

Anti-RAB38 antibody [EPR9427] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RAB38 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Ras-related protein Rab-38, Melanoma antigen NY-MEL-1, RAB38

1 Images
Western blot - Anti-RAB38 antibody [EPR9427] - BSA and Azide free (AB249211)
  • WB

Supplier Data

Western blot - Anti-RAB38 antibody [EPR9427] - BSA and Azide free (AB249211)

This data was developed using ab155956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB38 antibody [EPR9427] (<a href='/en-us/products/primary-antibodies/rab38-antibody-epr9427-ab155956'>ab155956</a>) at 1/1000 dilution

Lane 1:

Human melanoma lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9427

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249211 is the carrier-free version of ab155956.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB38 also known as Ras-related protein Rab-38 is a small GTPase involved in vesicular trafficking. It belongs to the RAB family containing proteins important for regulating intracellular vesicle transport. RAB38 has a molecular mass of approximately 25 kDa. This protein is predominantly expressed in lung and melanocytes suggesting its specific roles in these tissues. Its localization plays an important role in the transport and maturation of melanosomes which are organelles responsible for pigment production.
Biological function summary

RAB38 plays a significant role in melanosome biogenesis and function. It acts in concert with other proteins in a larger complex that includes melanophilin and other RAB family members such as RAB27A. This complex influences the transport of melanosomes along microtubules and their positioning within cells. The efficient functioning of RAB38 ensures the proper distribution and secretion of melanin which in turn affects pigmentation in tissues such as skin hair and eyes.

Pathways

RAB38 is involved in two key biological pathways: the lysosome biogenesis pathway and the endocytic recycling pathway. It works alongside proteins such as RAB7 and VPS11 in lysosome-related organelles which regulate endosomal trafficking processes. RAB38's function in these pathways is important for maintaining cellular homeostasis and ensuring the proper degradation and recycling of cellular components. Its role in these pathways demonstrates its interaction with other RAB proteins in modulating vesicle dynamics.

RAB38 has strong associations with Hermansky-Pudlak syndrome type 2 and pulmonary fibrosis. Hermansky-Pudlak syndrome type 2 is characterized by defects in lysosome-related organelles like melanosomes and platelet dense granules. In this disorder RAB38 interacts with proteins like AP3B1 playing a role in the abnormal biogenesis of lysosome-related organelles. In pulmonary fibrosis RAB38's dysregulation may contribute to fibrotic processes in the lung where its expression is notably high affecting processes like inflammation and tissue remodeling. These examples illustrate the involvement of RAB38 in various pathophysiological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in melanosomal transport and docking. Involved in the proper sorting of TYRP1. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with ANKRD27 and VAMP7 (By similarity). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed : 21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed : 23084991). In concert with RAB32, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).
See full target information Ras-related protein Rab-38
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

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