Rabbit Recombinant Monoclonal RAB39B antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (AB249132), expandable thumbnail](https://content.abcam.com/products/images/rab39b-antibody-epr9651-bsa-and-azide-free-ab249132--immunocytochemistry-immunofluorescence-img66171.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (AB249132)")
![Western blot - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (AB249132), expandable thumbnail](https://content.abcam.com/products/images/rab39b-antibody-epr9651-bsa-and-azide-free-ab249132--western-blot-img439426.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (AB249132)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Expected |
| Mouse | Tested | Expected | Not recommended | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Small GTPases Rab involved in autophagy (PubMed:27103069). The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:27103069). May regulate the homeostasis of SNCA/alpha-synuclein. Together with PICK1 proposed to ensure selectively GRIA2 exit from the endoplasmic reticulum to the Golgi and to regulate AMPAR compostion at the post-synapses and thus synaptic transmission (By similarity).
Alternative names
Ras-related protein Rab-39B, RAB39B
Recommended products
Rabbit Recombinant Monoclonal RAB39B antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Alternative names
Ras-related protein Rab-39B, RAB39B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR9651
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249132 is the carrier-free version of Anti-RAB39B antibody [EPR9651] ab154826.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
RAB39B also known as Ras-related protein Rab-39B is a member of the Rab family of small GTPases which has a molecular mass of approximately 26 kDa. RAB39B is expressed in several tissues but it shows higher expression levels in the brain. Mechanically RAB39B is involved in the regulation of intracellular vesicle trafficking specifically linked to endosome dynamics. This protein functions by switching between active GTP-bound and inactive GDP-bound states influencing membrane trafficking along actin and tubulin networks.
- Biological function summary
RAB39B plays a role in synapse function and maintenance. This protein associates with cellular membranes and is involved in the trafficking of AMPA receptors to the neuronal surface which impacts synaptic plasticity and neurotransmission. Although not typically described as part of a large complex RAB39B works in close association with other synaptic proteins to ensure proper synaptic function. Its function in neurons highlights the importance of precise vesicular trafficking in neurotransmitter release and synaptic strength.
- Pathways
RAB39B is integral to the endocytic trafficking and recycling pathways. It collaborates with key proteins like Rab7 and Rab5 in these cellular processes ensuring the proper sorting and recycling of synaptic vesicles. This precise control contributes to cellular homeostasis and efficient signal transmission at synapses which is critical for neuronal communication and overall brain function.
- Associated diseases and disorders
RAB39B is linked to neurodevelopmental disorders and has a significant connection to Parkinson's disease. Mutations in RAB39B can lead to intellectual disabilities and autism spectrum disorders. In the context of Parkinson's disease altered RAB39B function has been associated with the pathogenesis where it interacts with alpha-synuclein a protein significantly implicated in the disorder. These connections highlight the importance of RAB39B in maintaining neurological health and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132), expandable thumbnail](https://content.abcam.com/products/images/rab39b-antibody-epr9651-bsa-and-azide-free-ab249132--immunocytochemistry-immunofluorescence-img66171.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)")
Immunocytochemistry/ Immunofluorescence - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling RAB39B with Anti-RAB39B antibody [EPR9651] ab154826 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB39B antibody [EPR9651] ab154826).
![Western blot - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132), expandable thumbnail](https://content.abcam.com/products/images/rab39b-antibody-epr9651-bsa-and-azide-free-ab249132--western-blot-img439426.jpg "Western blot - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)")
Western blot - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)
This data was developed using the same antibody clone in a different buffer formulation (Anti-RAB39B antibody [EPR9651] ab154826).
Western blot: Rabbit Monoclonal[EPR9651] to RAB39B Anti-RAB39B antibody [EPR9651] ab154826 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 25 kDa in Wild-type U-87 MG UNBOILED cell lysates with no signal observed at this size in RAB39B knockout U-87 MG UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-RAB39B antibody [EPR9651] (Anti-RAB39B antibody [EPR9651] ab154826) at 1/1000 dilution
Lane 1: Wild-type U-87 MG UNBOILED at 20 µg
Lane 2: Western blot - Human RAB39B knockout U-87 MG cell line (Human RAB39B knockout U-87 MG cell line ab306767) at 20 µg
Lane 3: SH-SY5Y UNBOILED at 20 µg
Lane 4: Human Brain UNBOILED at 20 µg
Lane 5: K562 UNBOILED at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com