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Rabbit Recombinant Monoclonal RAB3A antibody. Suitable for IP, WB and reacts with Human, Recombinant full length protein - Human samples.

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Images

Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] (AB285163), expandable thumbnail
  • Western blot - Anti-Rab3A antibody [EPR24450-44] (AB285163), expandable thumbnail
  • Western blot - Anti-Rab3A antibody [EPR24450-44] (AB285163), expandable thumbnail
  • Western blot - Anti-Rab3A antibody [EPR24450-44] (AB285163), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant full length protein - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant full length protein - Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant full length protein - Human, Rat
Dilution info
-
Notes

-

Target data

Function

Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).

Alternative names

Ras-related protein Rab-3A, RAB3A

Recommended products

Rabbit Recombinant Monoclonal RAB3A antibody. Suitable for IP, WB and reacts with Human, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24450-44
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab3A also known as Ras-related protein Rab-3A acts as a small GTPase involved in regulating membrane trafficking. Its molecular mass is approximately 25 kDa. Rab3A is highly expressed in neurons particularly at synaptic vesicles. It cycles between an active GTP-bound state and an inactive GDP-bound state to regulate the docking and fusion of synaptic vesicles with the presynaptic membrane.

Biological function summary

Rab3A facilitates neurotransmitter release by interacting with effector proteins such as RIM and Rabphilin-3A. It is part of a larger complex that includes the SNAREs which help mediate synaptic vesicle fusion. Through its regulatory action Rab3A ensures that neurotransmitters are released in a controlled manner during synaptic transmission which is essential for proper neuronal communication.

Pathways

Rab3A participates in the synaptic vesicle cycle which is vital in neurotransmitter release. This cycle involves key proteins like Syntaxin and VAMP. Rab3A interacts with these SNARE proteins to participate in vesicle docking and priming. Additionally it plays a role in the calcium-dependent exocytosis pathway which relies on calcium ions triggering vesicle fusion at nerve terminals.

Associated diseases and disorders

Rab3A associates with neurological conditions such as epilepsy and synaptic dysfunction. Dysfunction in Rab3A mechanisms can lead to improper neurotransmitter release and synaptic connectivity contributing to these conditions. The protein links to diseases through its interactions with proteins such as RIM which can also affect synaptic function and are implicated in certain neurodevelopmental disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] (ab285163), expandable thumbnail

    Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] (ab285163)

    Rab3A was immunoprecipitated from 0.35 mg Human eyeball tissue lysate 10 ug with ab285163 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285163 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human eyeball tissue lysate 10 ug

    Lane 2: ab285163 IP in Human eyeball tissue lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab285163 in human eyeball tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 27 seconds

    All lanes: Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] (ab285163)

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163), expandable thumbnail

    Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 7.75 seconds

    His-tagged human RAB3A recombinant protein(aa1-220)
    His-tagged human RAB3B recombinant protein(aa1-219)
    His-tagged human RAB3C recombinant protein(aa1-227)
    His-tagged human RAB3D recombinant protein(aa1-219)

    A band around 55 kDa may be RAB3A dimer.

    All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163) at 1/1000 dilution

    Lane 1: His-tagged human RAB3A recombinant protein

    Lane 2: His-tagged human RAB3B recombinant protein

    Lane 3: His-tagged human RAB3C recombinant protein

    Lane 4: His-tagged human RAB3D recombinant protein

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163), expandable thumbnail

    Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: Human skeletal muscle (PMID: 11865063).

    Exposure time: 5.5 seconds

    All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate

    Lane 2: Human skeletal muscle tissue lysate

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163), expandable thumbnail

    Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163)

    ab285163 was shown to react with RAB3A in wild-type SKNFI cells in Western blot with loss of signal observed in a RAB3A knockout cell line. Wild-type SKNFI and RAB3A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab285163 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (ab285163) at 1/200 dilution

    Lane 1: Wild-type SKNFI lysate at 40 µg

    Lane 2: RAB3A knock-out SKNFI lysate at 40 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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