Rabbit Recombinant Monoclonal RAB3A antibody. Carrier free. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples.
Images
![Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--immunoprecipitation-img179370.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170)")
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img172106.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170)")
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img165345.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170)")
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img438538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (AB285170)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | Flow Cyt (Intra) | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).
Alternative names
Ras-related protein Rab-3A, RAB3A
Recommended products
Rabbit Recombinant Monoclonal RAB3A antibody. Carrier free. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24450-44
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab285170 is the carrier-free version of Anti-Rab3A antibody [EPR24450-44] ab285163.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rab3A also known as Ras-related protein Rab-3A acts as a small GTPase involved in regulating membrane trafficking. Its molecular mass is approximately 25 kDa. Rab3A is highly expressed in neurons particularly at synaptic vesicles. It cycles between an active GTP-bound state and an inactive GDP-bound state to regulate the docking and fusion of synaptic vesicles with the presynaptic membrane.
- Biological function summary
Rab3A facilitates neurotransmitter release by interacting with effector proteins such as RIM and Rabphilin-3A. It is part of a larger complex that includes the SNAREs which help mediate synaptic vesicle fusion. Through its regulatory action Rab3A ensures that neurotransmitters are released in a controlled manner during synaptic transmission which is essential for proper neuronal communication.
- Pathways
Rab3A participates in the synaptic vesicle cycle which is vital in neurotransmitter release. This cycle involves key proteins like Syntaxin and VAMP. Rab3A interacts with these SNARE proteins to participate in vesicle docking and priming. Additionally it plays a role in the calcium-dependent exocytosis pathway which relies on calcium ions triggering vesicle fusion at nerve terminals.
- Associated diseases and disorders
Rab3A associates with neurological conditions such as epilepsy and synaptic dysfunction. Dysfunction in Rab3A mechanisms can lead to improper neurotransmitter release and synaptic connectivity contributing to these conditions. The protein links to diseases through its interactions with proteins such as RIM which can also affect synaptic function and are implicated in certain neurodevelopmental disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--immunoprecipitation-img179370.jpg "Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)")
Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)
This data was developed using Anti-Rab3A antibody [EPR24450-44] ab285163, the same antibody clone in a different buffer formulation.
Rab3A was immunoprecipitated from 0.35 mg Human eyeball tissue lysate 10 ug with Anti-Rab3A antibody [EPR24450-44] ab285163 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rab3A antibody [EPR24450-44] ab285163 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human eyeball tissue lysate 10 ug
Lane 2: Anti-Rab3A antibody [EPR24450-44] ab285163 IP in Human eyeball tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Rab3A antibody [EPR24450-44] ab285163 in human eyeball tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 27 seconds
All lanes: Immunoprecipitation - Anti-Rab3A antibody [EPR24450-44] (Anti-Rab3A antibody [EPR24450-44] ab285163)
Predicted band size: 24 kDa
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img172106.jpg "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)")
Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)
This data was developed using Anti-Rab3A antibody [EPR24450-44] ab285163, the same antibody clone in a different buffer formulation.
Lane 1: His-tagged human RAB3A recombinant protein(aa1-220)
Lane 2: His-tagged human RAB3B recombinant protein(aa1-219)
Lane 3: His-tagged human RAB3C recombinant protein(aa1-227)
Lane 4: His-tagged human RAB3D recombinant protein(aa1-219)A band around 55 kDa may be RAB3A dimer.
All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (Anti-Rab3A antibody [EPR24450-44] ab285163) at 1/1000 dilution
Lane 1: His-tagged human RAB3A recombinant protein
Lane 2: His-tagged human RAB3B recombinant protein
Lane 3: His-tagged human RAB3C recombinant protein
Lane 4: His-tagged human RAB3D recombinant protein
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img165345.jpg "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)")
Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)
This data was developed using Anti-Rab3A antibody [EPR24450-44] ab285163, the same antibody clone in a different buffer formulation.
Negative control: Human skeletal muscle (PMID: 11865063).
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (Anti-Rab3A antibody [EPR24450-44] ab285163) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate
Lane 2: Human skeletal muscle tissue lysate
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 24 kDa
![Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170), expandable thumbnail](https://content.abcam.com/products/images/rab3a-antibody-epr24450-44-bsa-and-azide-free-ab285170--western-blot-img438538.jpg "Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)")
Western blot - Anti-Rab3A antibody [EPR24450-44] - BSA and Azide free (ab285170)
This data was developed using Anti-Rab3A antibody [EPR24450-44] ab285163, the same antibody clone in a different buffer formulation.
Anti-Rab3A antibody [EPR24450-44] ab285163 was shown to react with RAB3A in wild-type SKNFI cells in Western blot with loss of signal observed in a RAB3A knockout cell line. Wild-type SKNFI and RAB3A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Rab3A antibody [EPR24450-44] ab285163 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-Rab3A antibody [EPR24450-44] (Anti-Rab3A antibody [EPR24450-44] ab285163) at 1/200 dilution
Lane 1: Wild-type SKNFI lysate at 40 µg
Lane 2: RAB3A knock-out SKNFI lysate at 40 µg
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