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Rabbit Recombinant Monoclonal RAB3A phospho T86 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.

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Images

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (AB316304), expandable thumbnail
  • Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (AB316304), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotICC/IFFlow Cyt (Intra)
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).

Alternative names

Ras-related protein Rab-3A, RAB3A

Recommended products

Rabbit Recombinant Monoclonal RAB3A phospho T86 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJF-R46-4
Purification technique
Affinity purification Protein A
Specificity

This antibody has weak cross reaction with human RAB3B and RAB3C.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab3A also known as Ras-related protein Rab-3A acts as a small GTPase involved in regulating membrane trafficking. Its molecular mass is approximately 25 kDa. Rab3A is highly expressed in neurons particularly at synaptic vesicles. It cycles between an active GTP-bound state and an inactive GDP-bound state to regulate the docking and fusion of synaptic vesicles with the presynaptic membrane.

Biological function summary

Rab3A facilitates neurotransmitter release by interacting with effector proteins such as RIM and Rabphilin-3A. It is part of a larger complex that includes the SNAREs which help mediate synaptic vesicle fusion. Through its regulatory action Rab3A ensures that neurotransmitters are released in a controlled manner during synaptic transmission which is essential for proper neuronal communication.

Pathways

Rab3A participates in the synaptic vesicle cycle which is vital in neurotransmitter release. This cycle involves key proteins like Syntaxin and VAMP. Rab3A interacts with these SNARE proteins to participate in vesicle docking and priming. Additionally it plays a role in the calcium-dependent exocytosis pathway which relies on calcium ions triggering vesicle fusion at nerve terminals.

Associated diseases and disorders

Rab3A associates with neurological conditions such as epilepsy and synaptic dysfunction. Dysfunction in Rab3A mechanisms can lead to improper neurotransmitter release and synaptic connectivity contributing to these conditions. The protein links to diseases through its interactions with proteins such as RIM which can also affect synaptic function and are implicated in certain neurodevelopmental disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304), expandable thumbnail

    Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304)

    Goat anti-Rabbit IgG Secondary Antibody used at 1:25, 000.

    Anti-HA High Affinity from rat IgG1 used at 1:5000 (28KDa).

    Data was kindly provided by collaborator.

    Exposure time: Not provided.

    All lanes: Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304) at 1/1000 dilution

    Lane 1: HEK-293T cells transfected with a human RAB3A expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 2: Untreated HEK-293T cells transfected with a human RAB3A expression vector containing a HA-tag, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 3: HEK-293T cells transfected with a human RAB3B expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 4: Untreated HEK-293T cells transfected with a human RAB3B expression vector containing a HA-tag, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 5: HEK-293T cells transfected with a human RAB3C expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 6: Untreated HEK-293T cells transfected with a human RAB3C expression vector containing a HA-tag, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 7: HEK-293T cells transfected with a human RAB3D expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg with 5% BSA/TBST

    Lane 8: Untreated HEK-293T cells transfected with a human RAB3D expression vector containing a a HA-tag, whole cell lysate at 20 µg with 5% BSA/TBST

    Secondary

    All lanes: Goat anti-Rabbit IgG Secondary Antibody at 1/25000 dilution

    Observed band size: 28 kDa

  • Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304), expandable thumbnail

    Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304)

    Dot blot analysis of Rab3A (phospho T86) using ab316304 at 1:1000 (0.513 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    All lanes: Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (ab316304) at 1/1000 dilution

    Lane 1: Rab3A non-phospho peptide with 5% NFDM/TBST

    Lane 2: Rab3A (phospho T86) peptide a with 5% NFDM/TBST

    Lane 3: Rab3A (phospho T68) peptide b with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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