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AB316305

Anti-Rab3A (phospho T86) antibody [MJF-R46-4] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB3A phospho T86 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.

View Alternative Names

Ras-related protein Rab-3A, RAB3A

2 Images
Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] - BSA and Azide free (AB316305)
  • WB

Collaborator

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] - BSA and Azide free (AB316305)

This data was developed using ab316304, the same antibody clone in a different buffer formulation.

Goat anti-Rabbit IgG Secondary Antibody used at 1 : 25, 000.

Anti-HA High Affinity from rat IgG1 used at 1 : 5000 (28KDa).

Data was kindly provided by collaborator.

Exposure time : Not provided.

All lanes:

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (<a href='/en-us/products/primary-antibodies/rab3a-phospho-t86-antibody-mjf-r46-4-ab316304'>ab316304</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human RAB3A expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 2:

Untreated HEK-293T cells transfected with a human RAB3A expression vector containing a HA-tag, whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a human RAB3B expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 4:

Untreated HEK-293T cells transfected with a human RAB3B expression vector containing a HA-tag, whole cell lysate at 20 µg

Lane 5:

HEK-293T cells transfected with a human RAB3C expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 6:

Untreated HEK-293T cells transfected with a human RAB3C expression vector containing a HA-tag, whole cell lysate at 20 µg

Lane 7:

HEK-293T cells transfected with a human RAB3D expression vector containing a HA-tag treated with 100nM MLi-2 for 90 minutes, whole cell lysate at 20 µg

Lane 8:

Untreated HEK-293T cells transfected with a human RAB3D expression vector containing a a HA-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG Secondary Antibody at 1/25000 dilution

Observed band size: 28 kDa

false

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] - BSA and Azide free (AB316305)
  • WB

Supplier Data

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] - BSA and Azide free (AB316305)

This data was developed using ab316304, the same antibody clone in a different buffer formulation.

Dot blot analysis of Rab3A (phospho T86) using ab316304 at 1 : 1000 (0.513 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

All lanes:

Western blot - Anti-Rab3A (phospho T86) antibody [MJF-R46-4] (<a href='/en-us/products/primary-antibodies/rab3a-phospho-t86-antibody-mjf-r46-4-ab316304'>ab316304</a>) at 1/1000 dilution

Lane 1:

Rab3A non-phospho peptide

Lane 2:

Rab3A (phospho T86) peptide a

Lane 3:

Rab3A (phospho T68) peptide b

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

  • Unconjugated

    Anti-Rab3A (phospho T86) antibody [MJF-R46-4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R46-4

Isotype

IgG

Carrier free

Yes

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has weak cross reaction with human RAB3B and RAB3C.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab316305 is the carrirer-free version of ab316304.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab3A also known as Ras-related protein Rab-3A acts as a small GTPase involved in regulating membrane trafficking. Its molecular mass is approximately 25 kDa. Rab3A is highly expressed in neurons particularly at synaptic vesicles. It cycles between an active GTP-bound state and an inactive GDP-bound state to regulate the docking and fusion of synaptic vesicles with the presynaptic membrane.
Biological function summary

Rab3A facilitates neurotransmitter release by interacting with effector proteins such as RIM and Rabphilin-3A. It is part of a larger complex that includes the SNAREs which help mediate synaptic vesicle fusion. Through its regulatory action Rab3A ensures that neurotransmitters are released in a controlled manner during synaptic transmission which is essential for proper neuronal communication.

Pathways

Rab3A participates in the synaptic vesicle cycle which is vital in neurotransmitter release. This cycle involves key proteins like Syntaxin and VAMP. Rab3A interacts with these SNARE proteins to participate in vesicle docking and priming. Additionally it plays a role in the calcium-dependent exocytosis pathway which relies on calcium ions triggering vesicle fusion at nerve terminals.

Rab3A associates with neurological conditions such as epilepsy and synaptic dysfunction. Dysfunction in Rab3A mechanisms can lead to improper neurotransmitter release and synaptic connectivity contributing to these conditions. The protein links to diseases through its interactions with proteins such as RIM which can also affect synaptic function and are implicated in certain neurodevelopmental disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 2501306). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 2501306). RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (PubMed : 2501306). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed : 27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed : 22248876, PubMed : 30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity).
See full target information Ras-related protein Rab-3A phospho T86
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com