AB177949

Anti-RAB3B antibody [EPR12987]

Name: Anti-RAB3B antibody [EPR12987] (ab177949) | Abcam
Brand: Abcam Limited
SKU: AB177949
Price: 4405 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal RAB3B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Ras-related protein Rab-3B, RAB3B

2 Images

Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (AB177949)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (AB177949)

Intracellular flow cytometric analysis of permeabilized SW480 cellslabeling RAB3B with ab177949at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-RAB3B antibody [EPR12987] (AB177949)

All lanes:

Western blot - Anti-RAB3B antibody [EPR12987] (ab177949) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

Fetal brain lysate at 10 µg

Lane 4:

SW480 lysate at 10 µg

Predicted band size: 25 kDa

false

Carrier free

Anti-RAB3B antibody [EPR12987] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12987

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB3B is a small GTPase belonging to the RAB protein family known for its role in regulating vesicle trafficking. It is also referred to as Ras-related protein Rab-3B. The molecular mass of RAB3B is approximately 25 kDa. This protein is primarily expressed in the brain although it shows presence in other tissues as well. RAB3B functions by cycling between an active GTP-bound state and an inactive GDP-bound state facilitating the control of intracellular vesicle movement and positioning.

Biological function summary

RAB3B participates in synaptic vesicle exocytosis an important process in neurotransmitter release at synapses. It is part of the exocytotic machinery complex that coordinates vesicle docking and fusion with target membranes. This protein plays a significant role in ensuring effective neurotransmission which is important for neuronal communication. Given its role in synaptic function RAB3B contributes to the regulation of synaptic plasticity and memory formation.

Pathways

RAB3B is actively involved in the regulation of the synaptic vesicle cycle and exocytosis pathways. These pathways are important in neurotransmitter release and recycling in neurons. In the synaptic vesicle cycle RAB3B interacts with proteins like RAB3A and Munc-18 which play roles in vesicle docking and fusion. These interactions ensure the coordination and timing of neurotransmitter release which is essential for accurate neuronal signaling.

Mutations or dysregulation of RAB3B have been implicated in neurological conditions such as epilepsy and Alzheimer's disease. In epilepsy the disruption of normal synaptic vesicle trafficking can lead to abnormal neuronal excitability. Similarly in Alzheimer's disease altered vesicle transport associated with RAB3B may contribute to synaptic dysfunction and cognitive decline. RAB3B's interaction with other synaptic proteins becomes a critical factor in understanding its involvement in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 35871249). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 35871249).

See full target information Ras-related protein Rab-3B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 7:fcaf224 PubMed40574971

2025

Glycine-to-aspartic acid mutation at codon 51 in disrupts the synaptic localisation of α-synuclein and enhances its propensity for synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen West,Ammar Natalwala,Karamjit Singh Dolt,Douglas J Lamont,Melanie McMillan,Kelvin Luk,Tomoji Mashimo,Tilo Kunath

Translational andrology and urology 12:1497-1510 PubMed37969768

2023

Proteomic approach towards identification of seminal fluid biomarkers from individuals with severe oligozoospermia, cryptozoospermia and non-obstructive azoospermia: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Nowicka-Bauer,Marzena Kamieniczna,Marta Olszewska,Maciej Krzysztof Kurpisz

Cell death discovery 9:159 PubMed37173310

2023

N-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyang Qi,Chi Zhang,Huan Jian,Mengfan Hou,Yongfu Lou,Yi Kang,Wei Wang,Yigang Lv,Shenghui Shang,Chaoyu Wang,Xueying Li,Shiqing Feng,Hengxing Zhou

Journal of translational medicine 20:255 PubMed35668458

2022

RAF1 promotes lymphatic metastasis of hypopharyngeal carcinoma via regulating LAGE1: an experimental research.

Applications

Unspecified application

Species

Unspecified reactive species

Yanshi Li,Min Pan,Tao Lu,Dan Yu,Chuan Liu,Zhihai Wang,Guohua Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB3B antibody [EPR12987]

Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] (AB177949)

Western blot - Anti-RAB3B antibody [EPR12987] (AB177949)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise