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AB250020

Anti-RAB3B antibody [EPR12987] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB3B antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Ras-related protein Rab-3B, RAB3B

2 Images
Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] - BSA and Azide free (AB250020)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB3B antibody [EPR12987] - BSA and Azide free (AB250020)

This data was developed using ab177949, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SW480 cells labeling RAB3B with ab177949 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-RAB3B antibody [EPR12987] - BSA and Azide free (AB250020)
  • WB

Supplier Data

Western blot - Anti-RAB3B antibody [EPR12987] - BSA and Azide free (AB250020)

This data was developed using ab177949, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB3B antibody [EPR12987] (<a href='/en-us/products/primary-antibodies/rab3b-antibody-epr12987-ab177949'>ab177949</a>) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

Fetal brain lysate at 10 µg

Lane 4:

SW480 lysate at 10 µg

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12987

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250020 is the carrier-free version of ab177949.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB3B is a small GTPase belonging to the RAB protein family known for its role in regulating vesicle trafficking. It is also referred to as Ras-related protein Rab-3B. The molecular mass of RAB3B is approximately 25 kDa. This protein is primarily expressed in the brain although it shows presence in other tissues as well. RAB3B functions by cycling between an active GTP-bound state and an inactive GDP-bound state facilitating the control of intracellular vesicle movement and positioning.
Biological function summary

RAB3B participates in synaptic vesicle exocytosis an important process in neurotransmitter release at synapses. It is part of the exocytotic machinery complex that coordinates vesicle docking and fusion with target membranes. This protein plays a significant role in ensuring effective neurotransmission which is important for neuronal communication. Given its role in synaptic function RAB3B contributes to the regulation of synaptic plasticity and memory formation.

Pathways

RAB3B is actively involved in the regulation of the synaptic vesicle cycle and exocytosis pathways. These pathways are important in neurotransmitter release and recycling in neurons. In the synaptic vesicle cycle RAB3B interacts with proteins like RAB3A and Munc-18 which play roles in vesicle docking and fusion. These interactions ensure the coordination and timing of neurotransmitter release which is essential for accurate neuronal signaling.

Mutations or dysregulation of RAB3B have been implicated in neurological conditions such as epilepsy and Alzheimer's disease. In epilepsy the disruption of normal synaptic vesicle trafficking can lead to abnormal neuronal excitability. Similarly in Alzheimer's disease altered vesicle transport associated with RAB3B may contribute to synaptic dysfunction and cognitive decline. RAB3B's interaction with other synaptic proteins becomes a critical factor in understanding its involvement in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 35871249). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 35871249).
See full target information Ras-related protein Rab-3B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com