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Rabbit Recombinant Monoclonal RAB3D antibody. Carrier free. Suitable for mIHC, IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (AB248251), expandable thumbnail
  • Western blot - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (AB248251), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (AB248251), expandable thumbnail
  • OI-RD Scanning - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (AB248251), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (AB248251), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Protein transport. Probably involved in regulated exocytosis (By similarity).

Alternative names

Ras-related protein Rab-3D, RAB3D, GOV, RAB16

Recommended products

Rabbit Recombinant Monoclonal RAB3D antibody. Carrier free. Suitable for mIHC, IHC-P, WB and reacts with Human samples.

Alternative names

Ras-related protein Rab-3D, RAB3D, GOV, RAB16

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR8106

Purification technique

Affinity purification Protein A

Dissociation constant

3.36 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248251 is the carrier-free version of Anti-Rab3D antibody [EPR8106] ab128997.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rab3D also known as Ras-related protein Rab-3D belongs to the Rab family of small GTPases. It has a mass of approximately 25 kDa. Rab3D functions as a molecular switch that cycles between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking processes. This protein is highly expressed in secretory tissues such as exocrine pancreas salivary glands and osteoclasts where it plays a role in the secretory pathway.

Biological function summary

Rab3D plays an essential role in vesicle exocytosis modulating the secretion of enzymes hormones and other secretory products. Much of Rab3D's function depends on its interaction with other vesicle-associated proteins including Rab3-interacting molecules (RIMs) which are part of the secretory complex. Rab3D ensures proper docking and fusion of secretory vesicles with the plasma membrane.

Pathways

The Rab3D protein influences the exocytosis and endocytic pathways. It closely interacts with other members of the Rab family especially Rab27 and Rab8 which orchestrate various sub-tasks in vesicle trafficking. These pathways are vital for vesicle-mediated transport and require the regulated activity of Rab3D for proper cellular communication and secretion.

Associated diseases and disorders

Rab3D links to bone resorption disorders specifically related to osteoclast function and bone density diseases like osteoporosis. Dysregulation in Rab3D activity can influence osteoclast function where Rab3D works alongside proteins such as Rab27a contributing to the excessive bone resorption noted in osteoporosis. Additionally altered Rab3D function may have implications in disorders related to exocrine gland secretion impacting conditions like Sjögren's syndrome.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    This data was developed using Anti-Rab3D antibody [EPR8106] ab128997, the same antibody clone in a different buffer formulation.Anti-Rab3D antibody [EPR8106] ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    Western blot - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    This data was developed using Anti-Rab3D antibody [EPR8106] ab128997, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Rab3D antibody [EPR8106] (Anti-Rab3D antibody [EPR8106] ab128997) at 1/10000 dilution

    Lane 1: BxPC-3 cell lysate at 10 µg

    Lane 2: T.T cell lysate at 10 µg

    Lane 3: A549 cell lysate at 10 µg

    Lane 4: HT-29 cell lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

    Observed band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    This data was developed using Anti-Rab3D antibody [EPR8106] ab128997, the same antibody clone in a different buffer formulation.Anti-Rab3D antibody [EPR8106] ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    OI-RD Scanning - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Multiplex immunohistochemistry - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Chromogranin A (Anti-Chromogranin A antibody [EPR22537-249] ab254557, gray; Opal™690), anti-MUC-6 (Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Chromogranin A stained on neuroendocrine cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Chromogranin A antibody [EPR22537-249] ab254557 (1/5000), Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846 (1/1000), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

    This data was developed using Anti-Rab3D antibody [EPR8106] ab128997, the same antibody clone in a different buffer formulation.

  • Multiplex immunohistochemistry - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (Anti-Mucin 5AC antibody [45M1] ab3649, gray; Opal™690), anti-MUC-6 (Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Mucin 5AC antibody [45M1] ab3649 (1/5000 dilution), Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846 (1/1000 dilution), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

    This data was developed using Anti-Rab3D antibody [EPR8106] ab128997, the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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