AB133301

Anti-Rab3D antibody [EPR8107]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RAB3D antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

GOV, RAB16, RAB3D, Ras-related protein Rab-3D

2 Images

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Western blot - Anti-Rab3D antibody [EPR8107] (AB133301)

All lanes:

Western blot - Anti-Rab3D antibody [EPR8107] (ab133301) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

T.T cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Predicted band size: 24 kDa

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OI-RD Scanning - Anti-Rab3D antibody [EPR8107] (AB133301)

OI-RD Scanning

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OI-RD Scanning - Anti-Rab3D antibody [EPR8107] (AB133301)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8107

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab3D also known as Ras-related protein Rab-3D belongs to the Rab family of small GTPases. It has a mass of approximately 25 kDa. Rab3D functions as a molecular switch that cycles between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking processes. This protein is highly expressed in secretory tissues such as exocrine pancreas salivary glands and osteoclasts where it plays a role in the secretory pathway.

Biological function summary

Rab3D plays an essential role in vesicle exocytosis modulating the secretion of enzymes hormones and other secretory products. Much of Rab3D's function depends on its interaction with other vesicle-associated proteins including Rab3-interacting molecules (RIMs) which are part of the secretory complex. Rab3D ensures proper docking and fusion of secretory vesicles with the plasma membrane.

Pathways

The Rab3D protein influences the exocytosis and endocytic pathways. It closely interacts with other members of the Rab family especially Rab27 and Rab8 which orchestrate various sub-tasks in vesicle trafficking. These pathways are vital for vesicle-mediated transport and require the regulated activity of Rab3D for proper cellular communication and secretion.

Rab3D links to bone resorption disorders specifically related to osteoclast function and bone density diseases like osteoporosis. Dysregulation in Rab3D activity can influence osteoclast function where Rab3D works alongside proteins such as Rab27a contributing to the excessive bone resorption noted in osteoporosis. Additionally altered Rab3D function may have implications in disorders related to exocrine gland secretion impacting conditions like Sjögren's syndrome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein transport. Probably involved in regulated exocytosis (By similarity).

See full target information RAB3D

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 13:1090900 PubMed36698894

2023

Novel heterozygous mutation in suppressing the binding of amyloid-β (Aβ).

Applications

Unspecified application

Species

Unspecified reactive species

Guozhen Qiu,Liming Cao,Yong-Jun Chen

Cell cycle (Georgetown, Tex.) 21:1502-1511 PubMed35446199

2022

Linc01133 promotes proliferation and metastasis of human renal cell carcinoma through sponging miR-760.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lv,Yan Li,Liye Fu,Fandong Meng,Jun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab3D antibody [EPR8107]

Western blot - Anti-Rab3D antibody [EPR8107] (AB133301)

OI-RD Scanning - Anti-Rab3D antibody [EPR8107] (AB133301)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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