Anti-Rab4 antibody [EPR3043] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1 : 170 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109009).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109009).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)

ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109009).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1/200 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109009).

• IP

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Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)

ab109009 (purified) at 1 : 80 dilution (2�g) immunoprecipitating Rab4 in MCF7 whole cell lysate.
Lane 1 (input) : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10�g
Lane 2 (+) : ab109009 & MCF7 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109009 in MCF7 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109009).

All lanes:

Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (<a href='/en-us/products/primary-antibodies/rab4-antibody-epr3043-early-endosome-marker-ab109009'>ab109009</a>)

Predicted band size: 24 kDa

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