Rabbit Recombinant Monoclonal Rab4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img77084.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)")
![Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunoprecipitation-img4634.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)")
![Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--flow-cytometry-intracellular-img26570.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)")
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img126433.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)")
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img8630.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (AB226047)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Tested | Tested |
| Mouse | Predicted | Expected | Not recommended | Predicted | Predicted |
| Rat | Predicted | Expected | Not recommended | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed:15907487, PubMed:16034420). Involved in protein transport (PubMed:29425100). Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling (PubMed:29425100). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).
Alternative names
RAB4, RAB4A, Ras-related protein Rab-4A
Recommended products
Rabbit Recombinant Monoclonal Rab4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3043
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab226047 is the carrier-free version of Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rab4 also known as Ras-related protein Rab-4A is a small GTPase with a molecular mass of approximately 24 kDa. This protein is expressed in various cell types predominantly in tissues with high endocytic activity. Rab4 primarily functions as an endosome marker cooperating with the endocytic machinery to regulate sorting and recycling of endosomes. It occupies key positions on early endosomes relevant to its role in vesicular trafficking and membrane transportation. Endosome staining typically identifies Rab4 as an essential component in understanding endocytosis dynamics.
- Biological function summary
Rab4 coordinates the trafficking of cargo between endosomes and the plasma membrane. It usually forms part of a protein complex that governs the recycling pathway of internalized membrane proteins and lipids. Rab4 modulates various cellular processes by impacting the rapid recycling of receptors receptors often return to the cell surface in response to Rab4 regulation. This action is important for maintaining cellular homeostasis and efficient signal transduction serving as a checkpoint for receptor-mediated events.
- Pathways
The protein plays significant roles in the endocytic recycling pathway and the insulin signaling pathway. Rab4's activity influences the trafficking cycle often interacting with other proteins such as Rab11 and Rab5 to ensure efficient cargo movement within cells. By facilitating swift endosome recycling Rab4 has a direct impact on intracellular signaling and trafficking pathways supporting a range of cellular activities. This concerted interaction accentuates its importance in managing endosomal traffic and maintaining cellular connectivity.
- Associated diseases and disorders
Rab4 has implications in neurodegenerative disease and cancer. Abnormal Rab4 function disrupts endosomal signaling contributing to the pathogenesis of conditions like Alzheimer’s disease where it interacts with proteins such as amyloid precursor protein (APP). In cancer the dysregulation of Rab4-associated pathways affects tumor progression and metastasis. Rab4's connection with proteins like EGFR (Epidermal Growth Factor Receptor) indicates its potential role in altering cancer cell behaviors and resistance mechanisms making it a focus of interest in therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img77084.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)")
Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 at 1:170 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009).
![Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunoprecipitation-img4634.jpg "Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)")
Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)
Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 (purified) at 1:80 dilution (2�g) immunoprecipitating Rab4 in MCF7 whole cell lysate.
Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10�g
Lane 2 (+): Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 & MCF7 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 in MCF7 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009).All lanes: Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009)
Predicted band size: 24 kDa
![Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--flow-cytometry-intracellular-img26570.jpg "Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)")
Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 at 1/200 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009).
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img126433.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)")
Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009).
![Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047), expandable thumbnail](https://content.abcam.com/products/images/rab4-antibody-epr3043-bsa-and-azide-free-ab226047--immunocytochemistry-immunofluorescence-img8630.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)")
Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - BSA and Azide free (ab226047)
Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rab4 antibody [EPR3043] - Early Endosome Marker ab109009).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com