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Rabbit Recombinant Monoclonal Rab4 antibody. Early Endosome marker. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 11 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009), expandable thumbnail
  • Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009), expandable thumbnail
  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Tested
Not recommended
Expected
Expected
Rat
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/170 - 1/1000
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed:15907487, PubMed:16034420). Involved in protein transport (PubMed:29425100). Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling (PubMed:29425100). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).

Alternative names

RAB4, RAB4A, Ras-related protein Rab-4A

Recommended products

Rabbit Recombinant Monoclonal Rab4 antibody. Early Endosome marker. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3043
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab4 also known as Ras-related protein Rab-4A is a small GTPase with a molecular mass of approximately 24 kDa. This protein is expressed in various cell types predominantly in tissues with high endocytic activity. Rab4 primarily functions as an endosome marker cooperating with the endocytic machinery to regulate sorting and recycling of endosomes. It occupies key positions on early endosomes relevant to its role in vesicular trafficking and membrane transportation. Endosome staining typically identifies Rab4 as an essential component in understanding endocytosis dynamics.

Biological function summary

Rab4 coordinates the trafficking of cargo between endosomes and the plasma membrane. It usually forms part of a protein complex that governs the recycling pathway of internalized membrane proteins and lipids. Rab4 modulates various cellular processes by impacting the rapid recycling of receptors receptors often return to the cell surface in response to Rab4 regulation. This action is important for maintaining cellular homeostasis and efficient signal transduction serving as a checkpoint for receptor-mediated events.

Pathways

The protein plays significant roles in the endocytic recycling pathway and the insulin signaling pathway. Rab4's activity influences the trafficking cycle often interacting with other proteins such as Rab11 and Rab5 to ensure efficient cargo movement within cells. By facilitating swift endosome recycling Rab4 has a direct impact on intracellular signaling and trafficking pathways supporting a range of cellular activities. This concerted interaction accentuates its importance in managing endosomal traffic and maintaining cellular connectivity.

Associated diseases and disorders

Rab4 has implications in neurodegenerative disease and cancer. Abnormal Rab4 function disrupts endosomal signaling contributing to the pathogenesis of conditions like Alzheimer’s disease where it interacts with proteins such as amyloid precursor protein (APP). In cancer the dysregulation of Rab4-associated pathways affects tumor progression and metastasis. Rab4's connection with proteins like EGFR (Epidermal Growth Factor Receptor) indicates its potential role in altering cancer cell behaviors and resistance mechanisms making it a focus of interest in therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1:170 dilution (10 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    ab109009 (purified) at 1:80 dilution (2μg) immunoprecipitating Rab4 in MCF7 whole cell lysate.
    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab109009 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109009 in MCF7 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Rab4 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Human fetal brain lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Predicted band size: 24 kDa

  • Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1/200 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    All lanes: Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: PC12 cell lysate at 10 µg

    Lane 3: Neuro 2a cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Lane 5: SH SY5Y cell lysate at 10 µg

    Lane 6: Human fetal brain lysate at 10 µg

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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