AB58030

Anti-RAB43 antibody [5G4]

Name: Anti-RAB43 antibody [5G4] (ab58030) | Abcam
Brand: Abcam Limited
SKU: AB58030

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(4 Publications)

Mouse Monoclonal RAB43 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB43 aa 100-250.

View Alternative Names

RAB41, RAB43, Ras-related protein Rab-43, Ras-related protein Rab-41

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB43 antibody [5G4] (AB58030)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB43 antibody [5G4] (AB58030)

RAB43 antibody (ab58030) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-RAB43 antibody [5G4] (AB58030)

RAB43 antibody (ab58030) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RAB43 antibody [5G4] (ab58030)

Predicted band size: 23 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G4

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RAB43 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q86YS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 11^th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB43 also known as Ras-related protein Rab-43 is a member of the Ras superfamily of small GTPases specific for regulating intracellular vesicle trafficking. It has a molecular mass of about 25 kDa. RAB43 expresses in a variety of tissues with significant presence in the Golgi apparatus and endosomal compartments. These locations suggest its involvement in processes related to membrane trafficking and vesicular transport.

Biological function summary

The small GTPase RAB43 plays an important role in intracellular membrane trafficking and cargo sorting. It participates in a multi-protein complex that includes other small GTPases that help regulate membrane fusion events. RAB43 ensures accurate transport of proteins and lipids between the Golgi apparatus and various endosomal subcompartments. This regulation facilitates proper cellular function and maintenance of cellular architecture.

Pathways

Molecular studies have shown that RAB43 influences pathways such as endocytic trafficking and protein processing. It interacts with proteins involved in the vesicular transport pathway ensuring efficient transport of certain cargo molecules to their respective destinations. Notably RAB43 associates with proteins such as SNAREs within these pathways indicating its role in docking and fusion of transport vesicles.

Recent research highlights RAB43's involvement in neurodegenerative conditions such as Parkinson's disease and certain developmental disorders. Alterations in RAB43 expression or function disrupt vesicle trafficking potentially leading to neuronal damage. The interplay between RAB43 and proteins like LRRK2 which have significant links to Parkinson's disease supports its relevance in neurological health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

See full target information RAB43

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 45:1269-1277 PubMed33559954

2021

WTAP facilitates progression of endometrial cancer via CAV-1/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Li,Chenyu Wang,Wei Dong,Yi Su,Zhao Ma

Oncology reports 37:903-912 PubMed28075478

2017

High expression of RAB43 predicts poor prognosis and is associated with epithelial-mesenchymal transition in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Zhi Han,Bin Huang,An-Jing Chen,Xin Zhang,Ran Xu,Jian Wang,Xin-Gang Li

Scientific reports 6:23960 PubMed27044861

2016

Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Rhee,Seung-Hwan Lee,Ji-Won Kim,Dong-Sik Ham,Heon-Seok Park,Hae Kyung Yang,Ju-Young Shin,Jae-Hyoung Cho,Young-Bum Kim,Byung-Soo Youn,Hei Sook Sul,Kun-Ho Yoon

Molecular & cellular proteomics : MCP 13:3585-601 PubMed25277244

2014

The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets.

Applications

Species

Unspecified reactive species

Maria Katsogiannou,Claudia Andrieu,Virginie Baylot,Anaïs Baudot,Nelson J Dusetti,Odile Gayet,Pascal Finetti,Carmen Garrido,Daniel Birnbaum,François Bertucci,Christine Brun,Palma Rocchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB43 antibody [5G4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB43 antibody [5G4] (AB58030)

Western blot - Anti-RAB43 antibody [5G4] (AB58030)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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Product promise