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AB326413

Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB43 antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

RAB41, RAB43, Ras-related protein Rab-43, Ras-related protein Rab-41

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)

This data was developed using ab325751, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SR (human pleural effusion lymphoblast) and HeLa (human cervical adenocarcinoma epithelial cell) cells labelling RAB43 with ab325751 at 1/50 (10.34 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing golgi staining in SR cell line (shown in green), which is co-stained with a golgi marker. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Low expression : HeLa.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain golgi at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab325751 at 1/50 dilution, followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab169276 at 1/200 dilution, followed by ab150081 at 1/1000 dilution.

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)
  • WB

Lab

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)

This data was developed using ab325751, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HeLa, Daudi.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-RAB43 antibody [EPR30492-530] (<a href='/en-us/products/primary-antibodies/rab43-antibody-epr30492-530-ab325751'>ab325751</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 2:

SR (human pleural effusion lymphoblast) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 5:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)
  • WB

Lab

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)

This data was developed using ab325751, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-RAB43 antibody [EPR30492-530] (<a href='/en-us/products/primary-antibodies/rab43-antibody-epr30492-530-ab325751'>ab325751</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting RAB43 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)
  • WB

Lab

Western blot - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)

This data was developed using ab325751, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 5 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-RAB43 antibody [EPR30492-530] (<a href='/en-us/products/primary-antibodies/rab43-antibody-epr30492-530-ab325751'>ab325751</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 48 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 48 µg

Lane 3:

Mouse testis tissue lysate at 48 µg

Lane 4:

Mouse stomach tissue lysate at 48 µg

Lane 5:

Mouse brain tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

true

Exposure time: 180s

Indirect ELISA - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)
  • I-ELISA

Lab

Indirect ELISA - Anti-RAB43 antibody [EPR30492-530] - BSA and Azide free (AB326413)

This data was developed using ab325751, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab325751 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human RAB43 protein;Human RAB30 protein;Human RAB19 protein;Human RAB11A protein;Human RAB11B protein.

Antigen concentration : 1000 ng/ml

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30492-530

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with human RAB30, RAB19, RAB11A and RAB11B by ELISA. Unsuitable for mouse ICC

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab326413 is the carrier-free version of ab325751

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
See full target information RAB43
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com