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Rabbit Recombinant Monoclonal RAB45 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (AB194827), expandable thumbnail
  • Western blot - Anti-RAB45 antibody [EPR16349] (AB194827), expandable thumbnail
  • Western blot - Anti-RAB45 antibody [EPR16349] (AB194827), expandable thumbnail
  • Western blot - Anti-RAB45 antibody [EPR16349] (AB194827), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (AB194827), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Binds predominantly GDP, and also GTP (PubMed:17448446). Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules (PubMed:30814157).

Alternative names

RAB45, RASEF, Ras and EF-hand domain-containing protein, Ras-related protein Rab-45

Recommended products

Rabbit Recombinant Monoclonal RAB45 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16349
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB45 also known as RASEF is a member of the Ras superfamily of small GTPases. This protein has a molecular mass of approximately 83 kDa. It regulates various cellular processes by cycling between inactive GDP-bound and active GTP-bound states. RAB45 primarily localizes to intracellular membranes including endosomes and lysosomes. It is highly expressed in tissues such as the brain and pancreas indicating its role in specific cellular functions.

Biological function summary

RAB45 participates in intracellular trafficking ensuring proper vesicle flow and cargo delivery in the cell. It may associate with various effector proteins forming complexes needed for its function. Its role in membrane trafficking links it to pathways involving cellular signaling and homeostasis. RAB45 helps maintain cellular organization by managing membrane-bound compartments which is important for the effective function of endosomal and lysosomal pathways.

Pathways

RAB45 plays roles in vesicular transport and endocytic recycling processes. It interacts within the Rab family pathways that facilitate membrane trafficking. RAB45 is closely related to other Rab family proteins such as RAB7 and RAB9 which also regulate endosomal and lysosomal pathways. These interactions ensure coordination of intracellular transport and recycling processes necessary for cellular maintenance and function.

Associated diseases and disorders

RAB45 shows associations with certain neurological disorders and cancer types. Altered expression levels of RAB45 have been reported in glioblastoma suggesting its potential role in tumor progression. Additionally RAB45 might influence Alzheimer's disease by impacting vesicular pathways tied to APP processing. Connections with proteins such as tau in neurodegenerative pathways highlight the possible implications of RAB45 dysregulation in disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (ab194827), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (ab194827)

    Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed.

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827), expandable thumbnail

    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

    All lanes: Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Lane 6: C6 cell lysate at 10 µg

    Lane 7: RAW 264.7 cell lysate at 10 µg

    Lane 8: PC-12 cell lysate at 10 µg

    Lane 9: NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827), expandable thumbnail

    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

    All lanes: Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

    All lanes: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827), expandable thumbnail

    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

    All lanes: Western blot - Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution

    Lane 1: A549 cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Lane 3: Human testis lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa, 83 kDa

  • Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (ab194827), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] (ab194827)

    Intracellular Flow Cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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