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AB251195

Anti-RAB45 antibody [EPR16349] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RAB45 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

RAB45, RASEF, Ras and EF-hand domain-containing protein, Ras-related protein Rab-45

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm staining on HeLa cell line was observed.

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)
  • WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/en-us/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,83 kDa

false

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)
  • WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/en-us/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

RAW 264.7 cell lysate at 10 µg

Lane 8:

PC-12 cell lysate at 10 µg

Lane 9:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody : Blue. Rabbit monoclonal IgG was used as isotype control (Black).

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)
  • WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/en-us/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/10000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16349

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251195 is the carrier-free version of ab194827.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB45 also known as RASEF is a member of the Ras superfamily of small GTPases. This protein has a molecular mass of approximately 83 kDa. It regulates various cellular processes by cycling between inactive GDP-bound and active GTP-bound states. RAB45 primarily localizes to intracellular membranes including endosomes and lysosomes. It is highly expressed in tissues such as the brain and pancreas indicating its role in specific cellular functions.
Biological function summary

RAB45 participates in intracellular trafficking ensuring proper vesicle flow and cargo delivery in the cell. It may associate with various effector proteins forming complexes needed for its function. Its role in membrane trafficking links it to pathways involving cellular signaling and homeostasis. RAB45 helps maintain cellular organization by managing membrane-bound compartments which is important for the effective function of endosomal and lysosomal pathways.

Pathways

RAB45 plays roles in vesicular transport and endocytic recycling processes. It interacts within the Rab family pathways that facilitate membrane trafficking. RAB45 is closely related to other Rab family proteins such as RAB7 and RAB9 which also regulate endosomal and lysosomal pathways. These interactions ensure coordination of intracellular transport and recycling processes necessary for cellular maintenance and function.

RAB45 shows associations with certain neurological disorders and cancer types. Altered expression levels of RAB45 have been reported in glioblastoma suggesting its potential role in tumor progression. Additionally RAB45 might influence Alzheimer's disease by impacting vesicular pathways tied to APP processing. Connections with proteins such as tau in neurodegenerative pathways highlight the possible implications of RAB45 dysregulation in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds predominantly GDP, and also GTP (PubMed : 17448446). Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules (PubMed : 30814157).
See full target information RASEF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:1079-1090 PubMed37484809

2023

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfei Long,Yifei Ren,Qinglv Wei,Youchaou Mobet,Yujiao Liu,Hongyan Zhao,Tao Liu,Lei Cheng,Ping Yi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com