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Knockout Tested Mouse Recombinant Monoclonal RAB5A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (AB288775), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (AB288775), expandable thumbnail
  • Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (AB288775), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (AB288775), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (AB288775), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Alternative names

RAB5, RAB5A, Ras-related protein Rab-5A

Recommended products

Knockout Tested Mouse Recombinant Monoclonal RAB5A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
1/Rab5
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab288775 is the carrier-free version of Anti-Rab5 antibody [1/Rab5] ab288769

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.

Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Associated diseases and disorders

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    Performed under reducing conditions.

    False colour image of Western blot: Anti-Rab5 antibody (Anti-Rab5 antibody [1/Rab5] ab288769) staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Rab5 antibody [1/Rab5] ab288769 was shown to bind specifically to Rab5. A band was observed at 24 kDa in wild-type HAP1 cell lysates with no signal observed at this size in Rab5 knockout cell lysates. To generate this image, wild-type and Rab5 knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution.

    All lanes: Western blot - Anti-Rab5 antibody [1/Rab5] (Anti-Rab5 antibody [1/Rab5] ab288769) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: Rab5 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 24 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling Rab5 with Anti-Rab5 antibody [1/Rab5] ab288769 at 1/100 (8.35 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma.

    The section was incubated with Anti-Rab5 antibody [1/Rab5] ab288769 for 30 mins at room temperature and followed by specific mouse IgG2a antibody for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 70 seconds.

    All lanes: Western blot - Anti-Rab5 antibody [1/Rab5] (Anti-Rab5 antibody [1/Rab5] ab288769) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 24 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Rab5 with Anti-Rab5 antibody [1/Rab5] ab288769 at 1/100 (8.35 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human breast carcinoma. The section was incubated with Anti-Rab5 antibody [1/Rab5] ab288769 for 30 mins at room temperature and followed by specific mouse IgG2a antibody for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Rab5 with Anti-Rab5 antibody [1/Rab5] ab288769 at 1/100 (8.35 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human placenta. The section was incubated with Anti-Rab5 antibody [1/Rab5] ab288769 for 30 mins at room temperature and followed by specific mouse IgG2a antibody for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using Anti-Rab5 antibody [1/Rab5] ab288769 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Rab5 with Anti-Rab5 antibody [1/Rab5] ab288769 at 1/100 (8.35 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with Anti-Rab5 antibody [1/Rab5] ab288769 for 30 mins at room temperature and followed by specific mouse IgG2a antibody for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775), expandable thumbnail

    Western blot - Anti-Rab5 antibody [1/Rab5] - BSA and Azide free (ab288775)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Rab5 antibody [1/Rab5] ab288769)


    Anti-Rab5 antibody [1/Rab5] ab288769 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Rab5 antibody [1/Rab5] ab288769 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Rab5 antibody [1/Rab5] (Anti-Rab5 antibody [1/Rab5] ab288769) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 20 µg

    Lane 2: RAB5A knock-out HAP1 lysate at 20 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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