AB31261

Anti-Rab5 antibody - Drosophila Early Endosome Marker

Name: Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) | Abcam
Brand: Abcam Limited
SKU: AB31261
Rating: 5 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(73 Publications)

Rabbit Polyclonal Rab5 antibody. Early Endosome marker. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 73 publications.

1 Images

Ap465893****

Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (AB31261)

ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.

All lanes:

Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/mL

All lanes:

Schneider L2 whole cell lysate (<a href='/en-us/products/unavailable/schneider-l2-whole-cell-lysate-ab14893'>ab14893</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 24 kDa

Observed band size: 135 kDa,145 kDa,26 kDa

false

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.

Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rab5

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Publications (73)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx2893 PubMed40864718

2025

Mechanistic adaptation of the metazoan RabGEFs Mon1-Ccz1 and Fuzzy-Inturned.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan Wilmes,Jesse Tönjes,Maik Drechsler,Anita Ruf,Jan-Hannes Schäfer,Anna Lürick,Dovile Januliene,Steven Apelt,Daniele Di Iorio,Seraphine V Wegner,Martin Loose,Arne Moeller,Achim Paululat,Daniel Kümmel

Nature communications 14:7034 PubMed37923712

2023

Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Vanitha Nithianandam,Hassan Bukhari,Matthew J Leventhal,Rachel A Battaglia,Xianjun Dong,Ernest Fraenkel,Mel B Feany

Autophagy 20:769-791 PubMed37840274

2023

Removal of hypersignaling endosomes by simaphagy.

Applications

Unspecified application

Species

Unspecified reactive species

Simona M Migliano,Sebastian W Schultz,Eva M Wenzel,Szabolcs Takáts,Dan Liu,Silje Mørk,Kia Wee Tan,Tor Erik Rusten,Camilla Raiborg,Harald Stenmark

iScience 26:107335 PubMed37529104

2023

Role of Rab5 early endosomes in regulating gut antibacterial response.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Joshi,Annelise Viallat-Lieutaud,Julien Royet

Proceedings of the National Academy of Sciences of the United States of America 120:e2303750120 PubMed37463208

2023

Regulatory sites in the Mon1-Ccz1 complex control Rab5 to Rab7 transition and endosome maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Ann-Christin Borchers,Maren Janz,Jan-Hannes Schäfer,Arne Moeller,Daniel Kümmel,Achim Paululat,Christian Ungermann,Lars Langemeyer

Proceedings of the National Academy of Sciences of the United States of America 120:e2216887120 PubMed36920921

2023

Fragile X mental retardation protein coordinates neuron-to-glia communication for clearance of developmentally transient brain neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chunzhu Song,Kendal Broadie

Cellular and molecular life sciences : CMLS 80:24 PubMed36600084

2023

Rab26 controls secretory granule maturation and breakdown in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Attila Boda,Luca Petra Varga,Anikó Nagy,Győző Szenci,Tamás Csizmadia,Péter Lőrincz,Gábor Juhász

Cell reports 41:111583 PubMed36323251

2022

ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag.

Applications

Unspecified application

Species

Unspecified reactive species

Lingna Xu,Yunyi Qiu,Xufeng Wang,Weina Shang,Jian Bai,Kexin Shi,Hao Liu,Jun-Ping Liu,Liquan Wang,Chao Tong

The Journal of cell biology 222: PubMed36239632

2022

The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin E O'Brien,Susan H Younger,Lily Yeh Jan,Yuh Nung Jan

Journal of extracellular vesicles 11:e12263 PubMed36103151

2022

Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Linnemannstöns,Pradhipa Karuna M,Leonie Witte,Dolma Choezom,Mona Honemann-Capito,Alex Simon Lagurin,Chantal Vanessa Schmidt,Shreya Shrikhande,Lara-Kristin Steinmetz,Möbius Wiebke,Christof Lenz,Julia Christina Gross

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab5 antibody - Drosophila Early Endosome Marker

Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (AB31261)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (73)

Product promise