Rabbit Polyclonal RAB5A antibody. Suitable for ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human RAB5A aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Journal of innate immunity 15:581-5982023Regulation of Macrophage Cell Surface GAPDH Alters LL-37 Internalization and Downstream Effects in the Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsmita Dhiman,Sharmila Talukdar,Gaurav Kumar Chaubey,Rahul Dilawari,Radheshyam Modanwal,Surbhi Chaudhary,Anil Patidar,Vishant Mahendra Boradia,Pradeep Kumbhar,Chaaya Iyengar Raje,Manoj RajePubMed 37080180
- eLife 12:2023Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar Marcelo Lazo,Giampietro SchiavoPubMed 36897066
- Journal of virology 97:e01612222023Transferrin Receptor Protein 1 Is an Entry Factor for Rabies Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinxin Wang,Zhiyuan Wen,Huizhen Cao,Jie Luo,Lei Shuai,Chong Wang,Jinying Ge,Xijun Wang,Zhigao Bu,Jinliang WangPubMed 36779762
- Scientific reports 11:212032021Endocytic BDNF secretion regulated by Vamp3 in astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongho Han,Sungryeong Yoon,Hyungju ParkPubMed 34707216
- Frontiers in genetics 12:6767912021Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuya Kusama,Mohammad B Rashid,Rasoul Kowsar,Mohamed A Marey,Anup K Talukder,Kentaro Nagaoka,Masayuki Shimada,Hasan Khatib,Kazuhiko Imakawa,Akio MiyamotoPubMed 34262596
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:234-2502020Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarique R Bagalkot,Ethan R Block,Kristen Bucchin,Judith Joyce Balcita-Pedicino,Michael Calderon,Susan R Sesack,Alexander SorkinPubMed 33234607
- Cell death & disease 11:3142020Internalization mechanisms of brain-derived tau oligomers from patients with Alzheimer's disease, progressive supranuclear palsy and dementia with Lewy bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicha Puangmalai et. al.PubMed 32366836
- Molecular biology of the cell 30:2422-24342019Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristy Tower-Gilchrist et. al.PubMed 31268833
- Current biology : CB 29:775-789.e72019IPIP27 Coordinates PtdIns(4,5)P Homeostasis for Successful Cytokinesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrya C Carim et. al.PubMed 30799246
- PLoS biology 16:e20069512018Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonghai Tian et. al.PubMed 30481169
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 2.68% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAB5A aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P20339
Consider this alternative
Reactivity data
Select an application| ICC/IF | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Tested | Expected |
| Dog | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/80 | Notes - |
Species Human | Dilution info 1/80 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Use Bouin's fixative to fix tissue. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
Alternative names
RAB5, RAB5A, Ras-related protein Rab-5A
Recommended products
Rabbit Polyclonal RAB5A antibody. Suitable for ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human RAB5A aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 2.68% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAB5A aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P20339
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
- Biological function summary
Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.
- Pathways
The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.
- Associated diseases and disorders
Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceImmunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceImmunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)
ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7μm. Showing good co-localisation.
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)
Immunofluorescent analysis of 2% paraformaldehyde fixed Human cervical cancer cell line (HeLa) for 20min at RT, labelling Rab5 with ab13253 at 1/80 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody (green). (C) Composite image.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody (ab13253)
ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)
Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Rab5 with ab13253 at 1/80 dilution. Secondary antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
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