AB13253

Anti-Rab5 antibody - Early Endosome Marker

Name: Anti-Rab5 antibody - Early Endosome Marker (ab13253) | Abcam
Brand: Abcam Limited
SKU: AB13253
Price: 545 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(39 Publications)

Rabbit Polyclonal RAB5A antibody. Suitable for ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human RAB5A aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RAB5, RAB5A, Ras-related protein Rab-5A

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunofluorescent analysis of 2% paraformaldehyde fixed Human cervical cancer cell line (HeLa) for 20min at RT, labelling Rab5 with ab13253 at 1/80 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1 : 40000 for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody (green). (C) Composite image.

ICC/IF

AbReview3794****

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7μm. Showing good co-localisation.

This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Rab5 with ab13253 at 1/80 dilution. Secondary antibody : R-PE Goat Anti-Rabbit (yellow) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.

This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human RAB5A aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P20339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/80", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Use Bouin's fixative to fix tissue." }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/80", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.

Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

See full target information RAB5A

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:2011 PubMed39814913

2025

Recycling of endocytic BDNF through extracellular vesicles in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongho Han,Hyungju Park

Viruses 16: PubMed38675975

2024

Pathogenic and Apathogenic Strains of Lymphocytic Choriomeningitis Virus Have Distinct Entry and Innate Immune Activation Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan M Johnson,Nittaya Khakhum,Min Wang,Nikole L Warner,Jenny D Jokinen,Jason E Comer,Igor S Lukashevich

Journal of innate immunity 15:581-598 PubMed37080180

2023

Regulation of Macrophage Cell Surface GAPDH Alters LL-37 Internalization and Downstream Effects in the Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Asmita Dhiman,Sharmila Talukdar,Gaurav Kumar Chaubey,Rahul Dilawari,Radheshyam Modanwal,Surbhi Chaudhary,Anil Patidar,Vishant Mahendra Boradia,Pradeep Kumbhar,Chaaya Iyengar Raje,Manoj Raje

eLife 12: PubMed36897066

2023

Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Marcelo Lazo,Giampietro Schiavo

Journal of virology 97:e0161222 PubMed36779762

2023

Transferrin Receptor Protein 1 Is an Entry Factor for Rabies Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Wang,Zhiyuan Wen,Huizhen Cao,Jie Luo,Lei Shuai,Chong Wang,Jinying Ge,Xijun Wang,Zhigao Bu,Jinliang Wang

Frontiers in cellular and infection microbiology 12:1021657 PubMed36275033

2022

Vitamin D alters macrophage phenotype and endosomal trafficking markers in dairy cattle naturally infected with subsp. .

Applications

Unspecified application

Species

Unspecified reactive species

Taylor L T Wherry,Rohana P Dassanayake,John P Bannantine,Shankumar Mooyottu,Judith R Stabel

Scientific reports 11:21203 PubMed34707216

2021

Endocytic BDNF secretion regulated by Vamp3 in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongho Han,Sungryeong Yoon,Hyungju Park

Frontiers in genetics 12:676791 PubMed34262596

2021

Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Kusama,Mohammad B Rashid,Rasoul Kowsar,Mohamed A Marey,Anup K Talukder,Kentaro Nagaoka,Masayuki Shimada,Hasan Khatib,Kazuhiko Imakawa,Akio Miyamoto

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:234-250 PubMed33234607

2020

Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Tarique R Bagalkot,Ethan R Block,Kristen Bucchin,Judith Joyce Balcita-Pedicino,Michael Calderon,Susan R Sesack,Alexander Sorkin

Cell death & disease 11:314 PubMed32366836

2020

Internalization mechanisms of brain-derived tau oligomers from patients with Alzheimer's disease, progressive supranuclear palsy and dementia with Lewy bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Nicha Puangmalai,Nemil Bhatt,Mauro Montalbano,Urmi Sengupta,Sagar Gaikwad,Frank Ventura,Salome McAllen,Anna Ellsworth,Stephanie Garcia,Rakez Kayed

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab5 antibody - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody - Early Endosome Marker (AB13253)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

Product promise