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AB199530

Anti-Rab5 antibody [EPR17321] - Early Endosome Marker

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(6 Publications)

Rabbit Recombinant Monoclonal RAB5A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

RAB5, RAB5A, Ras-related protein Rab-5A

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1 : 500 dilution (2.2μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution (2μg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) ab195889 was used as a counterstain antibody at 1 : 200 dilution (2.5μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells.

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • WB

Lab

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (ab199530) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Rab5A protein (Tag Free) (<a href='/en-us/products/proteins-peptides/recombinant-human-rab5a-protein-ab62956'>ab62956</a>)

Lane 2:

Western blot - Recombinant Human Rab5b protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-rab5b-protein-ab104545'>ab104545</a>)

Lane 3:

Western blot - Recombinant Human RAB5C/RABL protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-rab5c-rabl-protein-ab156358'>ab156358</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • WB

Supplier Data

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (ab199530) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney) cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa

false

Exposure time: 10s

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • WB

Supplier Data

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (ab199530) at 1/1000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)
  • WB

Supplier Data

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (AB199530)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (ab199530) at 1/10000 dilution

All lanes:

U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Rab5 antibody [EPR17321] - BSA and Azide free

  • HRP

    HRP Anti-Rab5 antibody [EPR17321] - Early Endosome Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Rab5 antibody [EPR17321] - Early Endosome Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17321

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB218624

Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB218624)

  • 4
  • 2
Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

  • 5
  • 24
Primary Antibodies

AB128913

Anti-Rab11A antibody [EPR7587(B)]

RabMAb|Recombinant

Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (AB128913)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
See full target information RAB5A
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade5041 PubMed37043568

2023

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Tong,Sitong Zhang,Qiqi Liu,Chenyuan Huang,Haining Hao,Michelle Siying Tan,Xiaodong Yu,Charles Kang Liang Lou,Rong Huang,Zhe Zhang,Tongjie Liu,Pimin Gong,Cheng Han Ng,Mark Muthiah,Giorgia Pastorin,Matthias Gerhard Wacker,Xiaoyuan Chen,Gert Storm,Cheun Neng Lee,Lanwei Zhang,Huaxi Yi,Jiong-Wei Wang

Frontiers in cellular and infection microbiology 12:1019789 PubMed36389146

2022

The synthesis of extracellular vesicles by the protistan parasite .

Applications

Unspecified application

Species

Unspecified reactive species

Steven Santino Leonardi,Eileen Yiling Koh,Lei Deng,Chenyuan Huang,Lingjun Tong,Jiong-Wei Wang,Kevin Shyong-Wei Tan

Translational vision science & technology 11:15 PubMed35138344

2022

A Pilot Application of an iTRAQ-Based Proteomics Screen Estimates the Effects of Cigarette Smokers' Serum on RPE Cells With AMD High-Risk Alleles.

Applications

Unspecified application

Species

Unspecified reactive species

Bincui Cai,Zhe Zhang,Shuo Sun,Tingting Lin,Yifeng Ke,Zhiqing Li,Jin Yang,Xiaorong Li

Development (Cambridge, England) 148: PubMed34338279

2021

The exocyst complex regulates C. elegans germline stem cell proliferation by controlling membrane Notch levels.

Applications

Unspecified application

Species

Unspecified reactive species

Kumari Pushpa,Sunayana Dagar,Harsh Kumar,Diksha Pathak,Sivaram V S Mylavarapu

Journal of virology 94: PubMed32102886

2020

Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Miao Li,Xiuli Wang,Mengfei Sun,Cuiqing Ma,Wenzhang Liang,Xue Gao,Lin Wei

Traffic (Copenhagen, Denmark) 20:974-982 PubMed31503392

2019

Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paul T Manna,Luther J Davis,Margaret S Robinson
View all publications
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Product promise

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