AB66746

Anti-Rab5A antibody [3A4] - Early Endosome Marker

Name: Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) | Abcam
Brand: Abcam Limited
SKU: AB66746
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

KO Validated
Recombinant
Lab Essentials
What is this?

(1 Review)

(15 Publications)

Mouse Recombinant Monoclonal RAB5A antibody. Early Endosome marker. Suitable for WB and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus.

View Alternative Names

RAB5, RAB5A, Ras-related protein Rab-5A

4 Images

Lab

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Rab5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Hu brain whole cell lysate (20 μg)
Lane 4 : Ms brain whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

Predicted band size: 24 kDa

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Lab

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

ab66746 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab66746 overnight at 4 °C at a 1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

RAB5A Knockout HAP1 lysate at 20 µg

Observed band size: 24 kDa

false

Unknown

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

All lanes:

extracts of mouse brain at 45 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse

Predicted band size: 24 kDa

Observed band size: 24 kDa

true

Unknown

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

All lanes:

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

All lanes:

mouse brain at 45 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A4

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.

Biological function summary

Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.

Pathways

Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.

Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

See full target information RAB5A

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

PLoS pathogens 20:e1012256 PubMed39024394

2024

A genome-wide CRISPR/Cas9 knockout screen identifies TMEM239 as an important host factor in facilitating African swine fever virus entry into early endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Shen,Guigen Zhang,Xiaogang Weng,Renqiang Liu,Zhiheng Liu,Xiangpeng Sheng,Yuting Zhang,Yan Liu,Yanshuang Mu,Yuanmao Zhu,Encheng Sun,Jiwen Zhang,Fang Li,Changyou Xia,Junwei Ge,Zhonghua Liu,Zhigao Bu,Dongming Zhao

Nature communications 14:6190 PubMed37794006

2023

MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

He Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai Li

Science immunology 7:eadd0665 PubMed36563168

2022

Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley I Muendlein,Wilson M Connolly,James Cameron,David Jetton,Zoie Magri,Irina Smirnova,Edouard Vannier,Xudong Li,Amanda J Martinot,Rebecca Batorsky,Alexander Poltorak

Genetics in medicine : official journal of the American College of Medical Genetics 24:2399-2407 PubMed36083289

2022

Biallelic loss-of-function variants in RABGAP1 cause a novel neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Youjin Oh,Ashish R Deshwar,Ashish Marwaha,Nesrin Sabha,Michael Tropak,Huayun Hou,Kyoko E Yuki,Michael D Wilson,Patrick Rump,Roelineke Lunsing,Noha Elserafy,Clara W T Chung,Stacy Hewson,Tanja Klein-Rodewald,Julia Calzada-Wack,Adrián Sanz-Moreno,Markus Kraiger,Susan Marschall,Helmut Fuchs,Valerie Gailus-Durner,Martin Hrabe de Angelis,James Dowling,Andreas Schulze

Acta pharmaceutica Sinica. B 12:2043-2056 PubMed35847504

2022

The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyun Tang,Jinchao Gao,Tao Wang,Qian Zhang,Antian Wang,Meng Huang,Renhe Yu,Hongzhuan Chen,Xiaoling Gao

Frontiers in immunology 13:833515 PubMed35309340

2022

Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lam Ng,Xiaohui Wang,Chuanbin Yang,Chengfu Su,Min Li,Allen Ka Loon Cheung

British journal of cancer 126:1704-1714 PubMed35173303

2022

Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meera Patel,Kathryn A F Pennel,Jean A Quinn,Hannah Hood,David K Chang,Andrew V Biankin,Selma Rebus,Antonia K Roseweir,James H Park,Paul G Horgan,Donald C McMillan,Joanne Edwards

PLoS genetics 17:e1009551 PubMed34714826

2021

Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Huai-Wei Huang,Hyung Don Ryoo

Cell reports 35:109168 PubMed34038728

2021

Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Aikaterini Nanou,Mara Bourbouli,Stefania Vetrano,Ute Schaeper,Steven Ley,George Kollias

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rab5A antibody [3A4] - Early Endosome Marker

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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