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Mouse Monoclonal RAB5A antibody. Early Endosome marker. Suitable for WB and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus.


Images

Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746), expandable thumbnail
  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746), expandable thumbnail
  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746), expandable thumbnail
  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (AB66746), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20339

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Alternative names

RAB5, RAB5A, Ras-related protein Rab-5A

Recommended products

Mouse Monoclonal RAB5A antibody. Early Endosome marker. Suitable for WB and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human RAB5A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20339
Clone number

3A4

Purification technique

Affinity purification Protein G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.

Biological function summary

Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.

Pathways

Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.

Associated diseases and disorders

Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.

Product promise

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4 product images

  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746), expandable thumbnail

    Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: Rab5 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: Hu brain whole cell lysate (20 μg)
    Lane 4: Ms brain whole cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    This image was produced using the ascites version of this antibody.

    All lanes: Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746), expandable thumbnail

    Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

    All lanes: Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

    All lanes: mouse brain at 45 µg

    Secondary

    All lanes: goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746), expandable thumbnail

    Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

    This image was produced using the ascites version of this antibody.

    All lanes: Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution

    All lanes: extracts of mouse brain at 45 µg

    Secondary

    All lanes: HRP-conjugated goat anti-mouse

    Developed using the ECL technique.

    Predicted band size: 24 kDa

    Observed band size: 24 kDa

  • Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746), expandable thumbnail

    Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746)

    ab66746 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab66746 overnight at 4 °C at a 1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 20 µg

    Lane 2: RAB5A Knockout HAP1 lysate at 20 µg

    Observed band size: 24 kDa

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Product protocols

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