Rabbit Polyclonal RAB5A antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RAB5A.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAB5A. The exact immunogen used to generate this antibody is proprietary information. Database link P20339
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
Alternative names
RAB5, RAB5A, Ras-related protein Rab-5A
Recommended products
Rabbit Polyclonal RAB5A antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RAB5A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAB5A. The exact immunogen used to generate this antibody is proprietary information. Database link P20339
- Concentration
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.
- Biological function summary
Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.
- Pathways
Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.
- Associated diseases and disorders
Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-Rab5A antibody - C-terminal (ab228770)
Rab5 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab228770. Western blot was performed from the immunoprecipitate using ab228770 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell lysate 30 μg (Input).
Lane 2: Control IP with 2 μg of preimmune rabbit IgG in HeLa whole cell lysate.
Lane 3: ab228770 IP in HeLa whole cell lysate.
15% SDS-PAGE gel.
All lanes: Immunoprecipitation - Anti-Rab5A antibody - C-terminal (ab228770)
Predicted band size: 24 kDa
Western blot - Anti-Rab5A antibody - C-terminal (ab228770)
12% SDS-PAGE gel.
All lanes: Western blot - Anti-Rab5A antibody - C-terminal (ab228770) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa
Western blot - Anti-Rab5A antibody - C-terminal (ab228770)
All lanes: Western blot - Anti-Rab5A antibody - C-terminal (ab228770) at 1/1000 dilution
All lanes: Mouse brain lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa, 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5A antibody - C-terminal (ab228770)
Paraffin-embedded U-87 MG (human glioblastoma-astrocytoma epithelial cell line) xenograft stained for Rab5 using ab228770 at 1/500 dilution in immunohistochemical analysis.
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