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Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB6A.

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Images

Western blot - Anti-RAB6A antibody [HL1047] (AB308377), expandable thumbnail
  • Western blot - Anti-RAB6A antibody [HL1047] (AB308377), expandable thumbnail
  • Western blot - Anti-RAB6A antibody [HL1047] (AB308377), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (AB308377), expandable thumbnail
  • Western blot - Anti-RAB6A antibody [HL1047] (AB308377), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human RAB6A. Database link P20340

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Drosophila melanogaster
Expected
Expected
Tested
Zebrafish
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Rat, Zebrafish, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat, Zebrafish, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Zebrafish
Dilution info
1/1000
Notes

-

Species
Drosophila melanogaster
Dilution info
1/1000
Notes

-

Target data

Function

Regulator of COPI-independent retrograde transport from the Golgi apparatus towards the endoplasmic reticulum (ER) (PubMed:25962623). Has a low GTPase activity (PubMed:25962623). Recruits VPS13B to the Golgi membrane (PubMed:25492866). Plays a role in neuron projection development (Probable).

Alternative names

RAB6, RAB6A, Ras-related protein Rab-6A, Rab-6

Recommended products

Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB6A.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human RAB6A. Database link P20340
Carrier free
Yes
Clone number
HL1047
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab 6A also known as Ras-related protein Rab-6A is a member of the Rab family of small GTPases and has a molecular weight of approximately 24 kDa. This protein plays an essential role in intracellular transport mechanisms particularly overseeing the trafficking of proteins and lipids between the Golgi apparatus and the endoplasmic reticulum. Rab 6A is expressed in various tissues but is found predominantly in neuronal tissues where it has vital functions.

Biological function summary

This protein is involved in regulating membrane trafficking and maintains Golgi apparatus structure. Rab 6A engages with several other proteins to form complexes necessary for the vesicular transport processes. It aids in coordinating the interactions between transport vesicles and their target membranes directly impacting cellular homeostasis and protein export.

Pathways

Rab 6A operates significantly in the secretory pathway as well as the Golgi retrograde transport pathway. Within the secretory pathway it directly affects protein sorting and recycling. Additionally Rab 6A interacts with proteins such as Bicaudal-D and dynactin which link it to the recruitment and movement of vesicular cargo along the microtubules integrating it into the broader network of intracellular pathway regulation.

Associated diseases and disorders

Rab 6A has connections with neurological conditions. Its dysfunction or misregulation can impact neurodegenerative diseases such as Alzheimer's disease by disrupting protein processing and transport. Rab 6A interacts with amyloid precursor protein-processing pathways emphasizing its role in disease pathways. Also Rab 6A is linked to disorders affecting the Golgi apparatus a critical site for protein post-translational modification and trafficking within cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Western blot - Anti-RAB6A antibody [HL1047] (ab308377)

    All lanes: Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

    Lane 1: Wild-type (WT) 293T cell extract at 30 µg

    Lane 2: RAB6A knockout (KO) 293T cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 24 kDa

  • Western blot - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Western blot - Anti-RAB6A antibody [HL1047] (ab308377)

    All lanes: Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

    All lanes: Whole S2 cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 24 kDa

  • Western blot - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Western blot - Anti-RAB6A antibody [HL1047] (ab308377)

    Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (ab308377) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 50 µg

    Lane 2: Rat brain lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23.6 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (ab308377)

    Immunohistochemical analysis of mouse intestine tissue labeling RAB6A using ab308377 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Western blot - Anti-RAB6A antibody [HL1047] (ab308377)

    All lanes: Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

    All lanes: Whole zebrafish lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23.6 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB6A antibody [HL1047] (ab308377)

    For immunofluorescence analysis, A549 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of RAB6A protein using ab308377 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

  • Western blot - Anti-RAB6A antibody [HL1047] (ab308377), expandable thumbnail

    Western blot - Anti-RAB6A antibody [HL1047] (ab308377)

    Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (ab308377) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

    Lane 1: A549 cell lysate at 30 µg

    Lane 2: H1299 cell lysate at 30 µg

    Lane 3: HCT116 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23.6 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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