AB308377

Anti-RAB6A antibody [HL1047]

Recombinant
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Rabbit Recombinant Monoclonal RAB6A antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB6A.

View Alternative Names

RAB6, RAB6A, Ras-related protein Rab-6A, Rab-6

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-RAB6A antibody [HL1047] (AB308377)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB6A antibody [HL1047] (AB308377)

For immunofluorescence analysis, A549 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of RAB6A protein using ab308377 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (AB308377)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (AB308377)

Immunohistochemical analysis of mouse intestine tissue labeling RAB6A using ab308377 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

All lanes:

Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

Lane 1:

Wild-type (WT) 293T cell extract at 30 µg

Lane 2:

RAB6A knockout (KO) 293T cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (ab308377) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

Lane 1:

A549 cell lysate at 30 µg

Lane 2:

H1299 cell lysate at 30 µg

Lane 3:

HCT116 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23.6 kDa

false

Supplier Data

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (ab308377) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 50 µg

Lane 2:

Rat brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23.6 kDa

false

Supplier Data

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

All lanes:

Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

All lanes:

Whole S2 cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

All lanes:

Western blot - Anti-RAB6A antibody [HL1047] (ab308377) at 1/1000 dilution

All lanes:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23.6 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1047

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Zebrafish, Drosophila melanogaster

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAB6A.

P20340

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab 6A also known as Ras-related protein Rab-6A is a member of the Rab family of small GTPases and has a molecular weight of approximately 24 kDa. This protein plays an essential role in intracellular transport mechanisms particularly overseeing the trafficking of proteins and lipids between the Golgi apparatus and the endoplasmic reticulum. Rab 6A is expressed in various tissues but is found predominantly in neuronal tissues where it has vital functions.

Biological function summary

This protein is involved in regulating membrane trafficking and maintains Golgi apparatus structure. Rab 6A engages with several other proteins to form complexes necessary for the vesicular transport processes. It aids in coordinating the interactions between transport vesicles and their target membranes directly impacting cellular homeostasis and protein export.

Pathways

Rab 6A operates significantly in the secretory pathway as well as the Golgi retrograde transport pathway. Within the secretory pathway it directly affects protein sorting and recycling. Additionally Rab 6A interacts with proteins such as Bicaudal-D and dynactin which link it to the recruitment and movement of vesicular cargo along the microtubules integrating it into the broader network of intracellular pathway regulation.

Rab 6A has connections with neurological conditions. Its dysfunction or misregulation can impact neurodegenerative diseases such as Alzheimer's disease by disrupting protein processing and transport. Rab 6A interacts with amyloid precursor protein-processing pathways emphasizing its role in disease pathways. Also Rab 6A is linked to disorders affecting the Golgi apparatus a critical site for protein post-translational modification and trafficking within cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of COPI-independent retrograde transport from the Golgi apparatus towards the endoplasmic reticulum (ER) (PubMed : 25962623). Has a low GTPase activity (PubMed : 25962623). Recruits VPS13B to the Golgi membrane (PubMed : 25492866). Plays a role in neuron projection development (Probable).

See full target information RAB6A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB6A antibody [HL1047]

Immunocytochemistry/ Immunofluorescence - Anti-RAB6A antibody [HL1047] (AB308377)

Immunohistochemistry paraffin embedded sections - Anti-RAB6A antibody [HL1047] (AB308377)

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Western blot - Anti-RAB6A antibody [HL1047] (AB308377)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise