Rabbit Polyclonal RAB7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human RAB7A.
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- Cell 176:56-72.e152019Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Michel Cioni et. al.PubMed 30612743
- The EMBO journal 38:2018A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria R Villella,Andrea Venerando,Giorgio Cozza,Speranza Esposito,Eleonora Ferrari,Romina Monzani,Mara C Spinella,Vasilis Oikonomou,Giorgia Renga,Antonella Tosco,Federica Rossin,Stefano Guido,Marco Silano,Enrico Garaci,Yu-Kai Chao,Christian Grimm,Alessandro Luciani,Luigina Romani,Mauro Piacentini,Valeria Raia,Guido Kroemer,Luigi MaiuriPubMed 30498130
- Acta pharmacologica Sinica 40:724-7362018CID1067700, a late endosome GTPase Rab7 receptor antagonist, attenuates brain atrophy, improves neurologic deficits and inhibits reactive astrogliosis in rat ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Qin,Yang He,Yong-Ming Zhu,Min Li,Yong Ni,Jin Liu,Hui-Ling ZhangPubMed 30315251
- Cerebral cortex (New York, N.Y. : 1991) 29:1938-19522018Formin 2 Regulates Lysosomal Degradation of Wnt-Associated β-Catenin in Neural Progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGewei Lian,Anjen Chenn,Victor Ekuta,Sneha Kanaujia,Volney SheenPubMed 29659741
- Proceedings of the National Academy of Sciences of 114:E2327-E23362017Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayaka Saitoh et. al.PubMed 28270608
- Frontiers in molecular neuroscience 9:1302016Amyloid Precursor Proteins Are Dynamically Trafficked and Processed during Neuronal Development.Applications:ICC/IFReactive species:Unspecified reactive speciesJenna M Ramaker et. al.PubMed 27932950
- PloS one 11:e01605012016Mannose 6-Phosphate Receptor Is Reduced in -Synuclein Overexpressing Models of Parkinsons Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmela Matrone,Nicolas Dzamko,Peder Madsen,Mette Nyegaard,Regina Pohlmann,Rikke V Søndergaard,Louise B Lassen,Thomas L Andresen,Glenda M Halliday,Poul Henning Jensen,Morten S NielsenPubMed 27509067
- PloS one 11:e01575942016Evidence of Placental Autophagy during Early Pregnancy after Transfer of In Vitro Produced (IVP) Sheep Embryos.
- PloS one 10:e01418242015TECPR2 Associated Neuroaxonal Dystrophy in Spanish Water Dogs.
- Open biology 4:1400272014Autophagy and apoptosis: parent-of-origin genome-dependent mechanisms of cellular self-destruction.Applications:IHCReactive species:Unspecified reactive speciesGrazyna E Ptak,Paola Toschi,Antonella Fidanza,Marta Czernik,Federica Zacchini,Jacek A Modlinski,Pasqualino LoiPubMed 24898141
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAB7A. The exact immunogen used to generate this antibody is proprietary information. Database link P51149
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
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Target data
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Required for vesicular trafficking and cell surface expression of ACE2 (PubMed:33147445). May play a role in PRPH neuronal intermediate filament assembly (By similarity).
Alternative names
RAB7, RAB7A, Ras-related protein Rab-7a
Recommended products
Rabbit Polyclonal RAB7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human RAB7A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAB7A. The exact immunogen used to generate this antibody is proprietary information. Database link P51149
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB7 also known as RAB7A is a small GTPase belonging to the RAS oncogene family. It has a molecular mass of approximately 23 kDa. This protein plays an important role in the regulation of late endocytic trafficking. It is prominently expressed in the cytoplasmic compartments of various cell types and serves as an important late endosome marker. RAB7 controls the maturation of early endosomes into late endosomes and their subsequent fusion with lysosomes therefore influencing degradation pathways.
- Biological function summary
RAB7 affects various processes including autophagy cell signaling and pathogen infection response. It often functions as part of the endosomal machinery and forms complexes with other proteins like RAB3GAP1 and RILP. These complexes help mediate vesicle transport and membrane trafficking processes which are essential for the maintenance of cellular homeostasis. RAB7 is significant in ensuring proper lysosomal positioning and function which is critical for cellular metabolism and degradation.
- Pathways
RAB7 integrates into the endocytic and autophagic pathways by facilitating the transport between endosomes and lysosomes. It connects with the PI3K/AKT pathway impacting cell proliferation and survival. RAB7 also interacts with the retromer complex influencing the retrieval of receptors from endosomes. Proteins like FYCO1 and Prostaglandin E2 synthase can also cooperate with RAB7 in these pathways further establishing its role in cellular dynamics and signaling.
- Associated diseases and disorders
RAB7 has associations with Charcot-Marie-Tooth disease type 2B and cancer progression. Mutations in the RAB7 gene have been linked to neuropathic conditions leading to impaired motor and sensory function. In the context of cancer dysregulation of RAB7 expression can contribute to increased tumor growth and metastasis. RAB7 interacts with proteins such as MAPK and ARL8B which are involved in these diseases highlighting its importance in pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody (ab77993)
ab77993 at 10μg/ml staining RAB7 in human skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Western blot - Anti-RAB7 antibody (ab77993)
All lanes: Western blot - Anti-RAB7 antibody (ab77993) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 23 kDa, 34 kDa, 49 kDa
Exposure time: 1min
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