AB282214

Anti-RAB7 antibody [EPR24301-49] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal RAB7 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.

View Alternative Names

RAB7, RAB7A, Ras-related protein Rab-7a

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24301-49

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab282214 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat RAB7. The recommended pair for sandwich ELISA is:
Capture: ab282214, Human/Mouse/Rat RAB7 Capture Antibody (unconjugated)
Detector: ab282215, Human/Mouse/Rat RAB7 Detector Antibody (unconjugated)
The reference range value is 0.39 - 50 ng/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB7 also known as RAB7A is a small GTPase belonging to the RAS oncogene family. It has a molecular mass of approximately 23 kDa. This protein plays an important role in the regulation of late endocytic trafficking. It is prominently expressed in the cytoplasmic compartments of various cell types and serves as an important late endosome marker. RAB7 controls the maturation of early endosomes into late endosomes and their subsequent fusion with lysosomes therefore influencing degradation pathways.

Biological function summary

RAB7 affects various processes including autophagy cell signaling and pathogen infection response. It often functions as part of the endosomal machinery and forms complexes with other proteins like RAB3GAP1 and RILP. These complexes help mediate vesicle transport and membrane trafficking processes which are essential for the maintenance of cellular homeostasis. RAB7 is significant in ensuring proper lysosomal positioning and function which is critical for cellular metabolism and degradation.

Pathways

RAB7 integrates into the endocytic and autophagic pathways by facilitating the transport between endosomes and lysosomes. It connects with the PI3K/AKT pathway impacting cell proliferation and survival. RAB7 also interacts with the retromer complex influencing the retrieval of receptors from endosomes. Proteins like FYCO1 and Prostaglandin E2 synthase can also cooperate with RAB7 in these pathways further establishing its role in cellular dynamics and signaling.

RAB7 has associations with Charcot-Marie-Tooth disease type 2B and cancer progression. Mutations in the RAB7 gene have been linked to neuropathic conditions leading to impaired motor and sensory function. In the context of cancer dysregulation of RAB7 expression can contribute to increased tumor growth and metastasis. RAB7 interacts with proteins such as MAPK and ARL8B which are involved in these diseases highlighting its importance in pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed : 11179213, PubMed : 12944476, PubMed : 14617358, PubMed : 20028791, PubMed : 21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413). Required for vesicular trafficking and cell surface expression of ACE2 (PubMed : 33147445). May play a role in PRPH neuronal intermediate filament assembly (By similarity).

See full target information RAB7A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB7 antibody [EPR24301-49] - BSA and Azide free (Capture)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise