Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker

• WB

Supplier Data

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

All lanes:

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

NRK (rat cell kidney line) cell lysate

Lane 4:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 5:

MDCK (dog kidney cell line) cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-peroxidase

false

• WB

AbReview49734****

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

Blocked with 5% milk for 1 hour at 22°C.

Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

All lanes:

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution

All lanes:

Rat hepatocytes whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution

true

Exposure time: 30s

This image is courtesy of an Abreview submitted by Armen Petrosyan

• WB

AbReview20419****

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

Blocking Step : 5% Milk for 1 hour at 20°C

All lanes:

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/500 dilution

All lanes:

Human brain whole tissue lysate at 15 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution

Observed band size: 24 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

AbReview16518****

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

All lanes:

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/1000 dilution

All lanes:

Lysate prepared from mouse neuroblastoma cells at 10 µg

Secondary

All lanes:

HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution

Observed band size: 23 kDa

true

Exposure time: 20s

This image is a courtesy of Anonymous Abreview

• WB

AbReview49735****

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

Blocked with 5% milk for 1 hour at 22°C.

Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

All lanes:

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution

All lanes:

HepG2 whole cell lysate at 1/2000 dilution

Secondary

All lanes:

HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution

true

Exposure time: 30s

This image is courtesy of an Abreview submitted by Armen Petrosyan