Rabbit Recombinant Monoclonal RAB8A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 22 publications.
Images
![Western blot - Anti-RAB8A antibody [EPR14873] (AB188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img179.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (AB188574)")
![Western blot - Anti-RAB8A antibody [EPR14873] (AB188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img148635.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (AB188574)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (AB188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (AB188574)")
![Western blot - Anti-RAB8A antibody [EPR14873] (AB188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img2343.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (AB188574)")
![Western blot - Anti-RAB8A antibody [EPR14873] (AB188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img120811.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (AB188574)")
Publications
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- EMBO reports 23:e540902022ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Song,Yunfan Yang,Peng Zhou,Jie Ran,Liang Zhang,Xiaofan Wu,Wei Xie,Tao Zhong,Hongbin Liu,Min Liu,Dengwen Li,Huijie Zhao,Jun ZhouPubMed 35301795
- Frontiers in cell and developmental biology 10:8349642022Acrylamide Exposure Destroys the Distribution and Functions of Organelles in Mouse Oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao-Ying Zhao,Lin-Lin Hu,Chun-Hua Xing,Xiang Lu,Shao-Chen Sun,Yu-Xia Wei,Yan-Ping RenPubMed 35295848
- Nature protocols 16:5083-51222021Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWouter W Kallemeijn,Thomas Lanyon-Hogg,Nattawadee Panyain,Andrea Goya Grocin,Paulina Ciepla,Julia Morales-Sanfrutos,Edward W TatePubMed 34707257
- Cell 184:5089-5106.e212021Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah Scheiblich,Cira Dansokho,Dilek Mercan,Susanne V Schmidt,Luc Bousset,Lena Wischhof,Frederik Eikens,Alexandru Odainic,Jasper Spitzer,Angelika Griep,Stephanie Schwartz,Daniele Bano,Eicke Latz,Ronald Melki,Michael T HenekaPubMed 34555357
- Autophagy 18:204-2222021LRRK2 is required for CD38-mediated NAADP-Ca signaling and the downstream activation of TFEB (transcription factor EB) in immune cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeel R Nabar,Christopher N Heijjer,Chong-Shan Shi,Il-Young Hwang,Sundar Ganesan,Mikael C I Karlsson,John H KehrlPubMed 34313548
- Asian journal of pharmaceutical sciences 16:120-1282021Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Wang,Zui Zhang,Juan Guan,Weiyue Lu,Changyou ZhanPubMed 33613735
- Molecular & cellular proteomics : MCP 20:1000072020Quantitative Proteomics Reveals Association of Neuron Projection Development Genes ARF4, KIF5B, and RAB8A With Hirschsprung Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhang,Lihua Wu,Baoling Bai,Dan Li,Ping Xiao,Qi Li,Zhen Zhang,Hui Wang,Long Li,Qian JiangPubMed 33561610
- eLife 9:2020Pathway-specific dysregulation of striatal excitatory synapses by LRRK2 mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuyu Chen et. al.PubMed 33006315
- Neurobiology of disease 145:1050812020Roles of lysosomotropic agents on LRRK2 activation and Rab10 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoki Kuwahara et. al.PubMed 32919031
- Cells 9:2020Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPilar Rivero-Ríos et. al.PubMed 32709066
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/260 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Regulates the compacted morphology of the Golgi (PubMed:26209634). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation (PubMed:30209220). Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220).
Alternative names
MEL, RAB8, RAB8A, Ras-related protein Rab-8A, Oncogene c-mel
Recommended products
Rabbit Recombinant Monoclonal RAB8A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 22 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14873
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB8A is a small GTPase also referred to as Ras-related protein Rab-8A involved in intracellular membrane trafficking. It plays a critical role in the regulation of exocytosis the process by which cells release substances to the extracellular space. RAB8A mainly functions by cycling between an inactive GDP-bound form and an active GTP-bound form facilitating vesicle budding transport and fusion with target membranes. This protein is expressed in various tissues including the brain and pancreas where it assists in important cellular functions. RAB8A has a molecular mass of approximately 24 kDa.
- Biological function summary
The small GTPase RAB8A contributes to the biosynthetic transport from the trans-Golgi network to the plasma membrane. It performs this activity in coordination with other proteins as part of a larger complex. RAB8A also plays an essential role in cilia formation and maintenance affecting cell polarity and signaling functions. The protein's ability to regulate vesicle movement makes it important for cellular homeostasis and communication.
- Pathways
The regulatory function of RAB8A is significant in the ciliary membrane trafficking pathway and the insulin signaling pathway. Within these pathways RAB8A interacts closely with other proteins like Rab11 and Rabin8 which help support its roles in directing vesicle transport. These interactions are essential for proper cellular signaling and the maintenance of polarized cellular structures further supporting the function of various cellular pathways.
- Associated diseases and disorders
The dysfunction of RAB8A has associations with certain ciliopathies notably Bardet-Biedl syndrome. This condition involves abnormalities in cilia structure and function leading to symptoms like kidney disease vision impairment and obesity. Moreover disruptions in RAB8A have connections with insulin resistance implicating it in metabolic disorders. In the context of these diseases proteins such as BBS proteins and Rab11 play a role in mediating the effects of dysfunctional RAB8A-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img179.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
Lanes 1-4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab188574 Anti-RAB8A antibody [EPR14873] was shown to specifically react with RAB8A in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human RAB8A knockout HeLa cell line ab264993 (knockout cell lysate Human RAB8A knockout HeLa cell lysate ab257195) was used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RAB8A knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human RAB8A knockout HeLa cell line (Human RAB8A knockout HeLa cell line ab264993)
Lane 3: HCT116 cell lysate at 20 µg
Lane 4: Human brain tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img148635.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
Lanes 1 - 4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab188574 was shown to specifically react with RAB8A when RAB8A knockout samples were used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: RAB8A knockout HAP1 cell lysate at 20 µg
Lane 3: HCT116 cell lysate at 20 µg
Lane 4: NIH/3T3 cell lysate at 20 µg
Predicted band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labelling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img2343.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
Lanes 1-4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab188574 Anti-RAB8A antibody [EPR14873] was shown to specifically react with RAB8A in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human RAB8A knockout HeLa cell line ab264993 (knockout cell lysate Human RAB8A knockout HeLa cell lysate ab257195) was used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RAB8A knockout HeLa cell lysate at 20 µg
Lane 3: HCT116 cell lysate at 20 µg
Lane 4: Human brain tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img120811.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution
Lane 1: C6 lysate at 10 µg
Lane 2: RAW264.7 lysate at 10 µg
Lane 3: PC-12 lysate at 10 µg
Lane 4: NIH3T3 lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img68081.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution
All lanes: HCT-116 lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img105790.jpg "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)
All lanes: Western blot - Anti-RAB8A antibody [EPR14873] (ab188574) at 1/10000 dilution
Lane 1: HepG2 lysate at 20 µg
Lane 2: HeLa lysate at 20 µg
Lane 3: 293T lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Flow Cytometry (Intracellular) - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--flow-cytometry-intracellular-img74572.jpg "Flow Cytometry (Intracellular) - Anti-RAB8A antibody [EPR14873] (ab188574)")
Flow Cytometry (Intracellular) - Anti-RAB8A antibody [EPR14873] (ab188574)
Intracellular flow cytometrical analysis of HeLa cells labeling RAB8A with ab188574 at 1/260 (pink) compared to a Isotype control (green). FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunocytochemistry-immunofluorescence-img14125.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574)")
Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labelling RAB8A with ab188574 at 1/500 followed by Goat anti rabbit IgG (Alexa Fluor® 488) and DAPI staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135435.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img68081.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
![Western blot - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--western-blot-img105790.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)")
![Flow Cytometry (Intracellular) - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--flow-cytometry-intracellular-img74572.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RAB8A antibody [EPR14873] (ab188574)")
![Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunocytochemistry-immunofluorescence-img14125.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574), expandable thumbnail](https://content.abcam.com/products/images/rab8a-antibody-epr14873-ab188574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135435.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)")
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