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AB259754

Anti-RAB8A antibody [EPR14873] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal RAB8A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

MEL, RAB8, RAB8A, Ras-related protein Rab-8A, Oncogene c-mel

1 Images
Sandwich ELISA - Anti-RAB8A antibody [EPR14873] - BSA and Azide free (Detector) (AB259754)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RAB8A antibody [EPR14873] - BSA and Azide free (Detector) (AB259754)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255718).

  • Carrier free

    Anti-RAB8A antibody [EPR14873] - Low endotoxin, Azide free

  • Carrier free

    Anti-RAB8A antibody [EPR14873] - BSA and Azide free

  • Unconjugated

    Anti-RAB8A antibody [EPR14873]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-RAB8A antibody [EPR14873] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-RAB8A antibody [EPR14873] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RAB8A antibody [EPR14873] - C-terminal

  • HRP

    HRP Anti-RAB8A antibody [EPR14873] - C-terminal

  • 578 PE

    PE Anti-RAB8A antibody [EPR14873] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14873

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB255718

Human RAB8 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human RAB8 ELISA Kit (AB255718)

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Primary Antibodies

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Primary Antibodies

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Anti-RAB7 (phospho S72) antibody [MJF-R38-1]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 (phospho S72) antibody [MJF-R38-1] (AB302494)

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Primary Antibodies

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Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab259754 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RAB8. The recommended pair for sandwich ELISA is:
Capture: ab259753, Human RAB8 Capture Antibody (unconjugated)
Detector: ab259754, Human RAB8 Detector Antibody (unconjugated)
The reference range value is 0.09 - 6 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB8A is a small GTPase also referred to as Ras-related protein Rab-8A involved in intracellular membrane trafficking. It plays a critical role in the regulation of exocytosis the process by which cells release substances to the extracellular space. RAB8A mainly functions by cycling between an inactive GDP-bound form and an active GTP-bound form facilitating vesicle budding transport and fusion with target membranes. This protein is expressed in various tissues including the brain and pancreas where it assists in important cellular functions. RAB8A has a molecular mass of approximately 24 kDa.
Biological function summary

The small GTPase RAB8A contributes to the biosynthetic transport from the trans-Golgi network to the plasma membrane. It performs this activity in coordination with other proteins as part of a larger complex. RAB8A also plays an essential role in cilia formation and maintenance affecting cell polarity and signaling functions. The protein's ability to regulate vesicle movement makes it important for cellular homeostasis and communication.

Pathways

The regulatory function of RAB8A is significant in the ciliary membrane trafficking pathway and the insulin signaling pathway. Within these pathways RAB8A interacts closely with other proteins like Rab11 and Rabin8 which help support its roles in directing vesicle transport. These interactions are essential for proper cellular signaling and the maintenance of polarized cellular structures further supporting the function of various cellular pathways.

The dysfunction of RAB8A has associations with certain ciliopathies notably Bardet-Biedl syndrome. This condition involves abnormalities in cilia structure and function leading to symptoms like kidney disease vision impairment and obesity. Moreover disruptions in RAB8A have connections with insulin resistance implicating it in metabolic disorders. In the context of these diseases proteins such as BBS proteins and Rab11 play a role in mediating the effects of dysfunctional RAB8A-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB8A is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed : 20890297). Regulates the compacted morphology of the Golgi (PubMed : 26209634). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed : 21282656). Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed : 21844891, PubMed : 30398148, PubMed : 20631154). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed : 27103069). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed : 32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation (PubMed : 30209220). Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins (PubMed : 30209220).
See full target information RAB8A
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Product promise

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For full details, please see our Terms & Conditions

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