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Mouse Recombinant Monoclonal RAB8A antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (AB307607), expandable thumbnail
  • Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (AB307607), expandable thumbnail
  • Western blot - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (AB307607), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (AB307607), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (AB307607), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/800
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Regulates the compacted morphology of the Golgi (PubMed:26209634). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation (PubMed:30209220). Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220).

Alternative names

MEL, RAB8, RAB8A, Ras-related protein Rab-8A, Oncogene c-mel

Recommended products

Mouse Recombinant Monoclonal RAB8A antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJF-R22-79-3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab307607 is a mouse monoclonal chimeric antibody.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RAB8A is a small GTPase also referred to as Ras-related protein Rab-8A involved in intracellular membrane trafficking. It plays a critical role in the regulation of exocytosis the process by which cells release substances to the extracellular space. RAB8A mainly functions by cycling between an inactive GDP-bound form and an active GTP-bound form facilitating vesicle budding transport and fusion with target membranes. This protein is expressed in various tissues including the brain and pancreas where it assists in important cellular functions. RAB8A has a molecular mass of approximately 24 kDa.

Biological function summary

The small GTPase RAB8A contributes to the biosynthetic transport from the trans-Golgi network to the plasma membrane. It performs this activity in coordination with other proteins as part of a larger complex. RAB8A also plays an essential role in cilia formation and maintenance affecting cell polarity and signaling functions. The protein's ability to regulate vesicle movement makes it important for cellular homeostasis and communication.

Pathways

The regulatory function of RAB8A is significant in the ciliary membrane trafficking pathway and the insulin signaling pathway. Within these pathways RAB8A interacts closely with other proteins like Rab11 and Rabin8 which help support its roles in directing vesicle transport. These interactions are essential for proper cellular signaling and the maintenance of polarized cellular structures further supporting the function of various cellular pathways.

Associated diseases and disorders

The dysfunction of RAB8A has associations with certain ciliopathies notably Bardet-Biedl syndrome. This condition involves abnormalities in cilia structure and function leading to symptoms like kidney disease vision impairment and obesity. Moreover disruptions in RAB8A have connections with insulin resistance implicating it in metabolic disorders. In the context of these diseases proteins such as BBS proteins and Rab11 play a role in mediating the effects of dysfunctional RAB8A-related pathways.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized RAB8A KO HeLa (ED010027, Left) / Parental HeLa (EDWT01, Right) cells labeling RAB8A with ab307607 at 1/800 dilution (0.1 ug) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    RAB8A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug with ab307607 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307607 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A549 whole cell lysate 10 ug

    Lane 2: ab307607 IP in A549 whole cell lysate

    Lane 3: Mouse IgG2a monoclonal isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab307607 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds.

    All lanes: Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: A549 whole cell lysate

    Lane 3: Immunoprecipitation - Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413)

    Observed band size: 23 kDa

    Exposure time: 24s

  • Western blot - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Western blot - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, ab307607 was shown to bind specifically to RAB8A. A band was observed at 23 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in RAB8A knockout cell line Human RAB8A knockout HeLa cell line ab264993 (knockout cell lysate Human RAB8A knockout HeLa cell lysate ab257195).

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607) at 1/5000 dilution

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg

    Lane 2: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg

    Lane 3: HCT116 (human colorectal carcinoma epithelial cell)whole cell lysate 20 μg

    Lane 4: A549 (human lung carcinoma epithelial cell), whole cell lysate 20 μg

    Observed band size: 23 kDa

    Exposure time: 48s

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
    Confocal image showing cytoplasmic staining in A549 cell line.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/50 dilution (10 ug/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAB8A KO HeLa (RAB8A knockout human cervical adenocarcinoma epithelial cell) (Human RAB8A knockout HeLa cell line ab264993) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
    Confocal image showing cytoplasmic staining in Parental HeLa cell line, and no staining in RAB8A KO HeLa cell line.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/50 dilution (10 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

  • Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Immunohistochemical analysis of paraffin-embedded cell pellets labeling RAB8A with ab307607 at 1/5000 dilution (0.167 µg/mL) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining on (A) Wild-type HeLa cell pellets, no staining on (B) RAB8A knockout HeLa (Human RAB8A knockout HeLa cell line ab264993) cell pellets.
    The section was incubated with ab307607 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling RAB8A with ab307607 at 1/5000 dilution (0.167 µg/mL) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining on human bladder carcinoma.
    The section was incubated with ab307607 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607), expandable thumbnail

    Immunohistochemistry - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (ab307607)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling RAB8A with ab307607 at 1/5000 dilution (0.167 µg/mL) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining on human breast.
    The section was incubated with ab307607 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

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Product protocols

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