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Rabbit Recombinant Monoclonal RAB8A phospho S111 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (AB267492), expandable thumbnail
  • Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (AB267492), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). May be involved in ciliogenesis (PubMed:21844891, PubMed:30398148). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069).

Alternative names

Ras-related protein Rab-8A, Oncogene c-mel, RAB8A, RAB8, MEL

Recommended products

Rabbit Recombinant Monoclonal RAB8A phospho S111 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

Alternative names

Ras-related protein Rab-8A, Oncogene c-mel, RAB8A, RAB8, MEL

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

MJF-R27-30

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody was developed with support from The Michael J. Fox Foundation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492), expandable thumbnail

    Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.

    The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.

    Exposure time: 3min

    All lanes: Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO at 20 µg

    Lane 2: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP at 20 µg

    Lane 3: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO at 20 µg

    Lane 4: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa

    Observed band size: 23 kDa

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.

    The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.

    Exposure time: 3min

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Recombinant Human STAT1 protein (Tagged) ab276492).

  • Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492), expandable thumbnail

    Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492)

    10 ug/well NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate.
    Anti-RAB8A (phospho S111) antibody (ab267492) and Anti-RAB8A antibody (Anti-RAB8A antibody [EPR14873] ab188574) at 1/1000 dilution.
    Gel: 4-20% TGX gel
    Blocking buffer: 5% BSA blocking buffer

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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