Rabbit Recombinant Monoclonal RAB8A phospho S111 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Images
![Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (AB267493), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-s111-antibody-mjf-r27-30-bsa-and-azide-free-ab267493--western-blot-img5261.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (AB267493)")
![Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (AB267493), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-s111-antibody-mjf-r27-30-bsa-and-azide-free-ab267493--western-blot-img402591.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (AB267493)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Regulates the compacted morphology of the Golgi (PubMed:26209634). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed:32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation (PubMed:30209220). Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins (PubMed:30209220).
Alternative names
MEL, RAB8, RAB8A, Ras-related protein Rab-8A, Oncogene c-mel
Recommended products
Rabbit Recombinant Monoclonal RAB8A phospho S111 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- MJF-R27-30
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab267493 is the carrier-free version of Anti-RAB8A (phospho S111) antibody [MJF-R27-30] ab267492.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB8A is a small GTPase also referred to as Ras-related protein Rab-8A involved in intracellular membrane trafficking. It plays a critical role in the regulation of exocytosis the process by which cells release substances to the extracellular space. RAB8A mainly functions by cycling between an inactive GDP-bound form and an active GTP-bound form facilitating vesicle budding transport and fusion with target membranes. This protein is expressed in various tissues including the brain and pancreas where it assists in important cellular functions. RAB8A has a molecular mass of approximately 24 kDa.
- Biological function summary
The small GTPase RAB8A contributes to the biosynthetic transport from the trans-Golgi network to the plasma membrane. It performs this activity in coordination with other proteins as part of a larger complex. RAB8A also plays an essential role in cilia formation and maintenance affecting cell polarity and signaling functions. The protein's ability to regulate vesicle movement makes it important for cellular homeostasis and communication.
- Pathways
The regulatory function of RAB8A is significant in the ciliary membrane trafficking pathway and the insulin signaling pathway. Within these pathways RAB8A interacts closely with other proteins like Rab11 and Rabin8 which help support its roles in directing vesicle transport. These interactions are essential for proper cellular signaling and the maintenance of polarized cellular structures further supporting the function of various cellular pathways.
- Associated diseases and disorders
The dysfunction of RAB8A has associations with certain ciliopathies notably Bardet-Biedl syndrome. This condition involves abnormalities in cilia structure and function leading to symptoms like kidney disease vision impairment and obesity. Moreover disruptions in RAB8A have connections with insulin resistance implicating it in metabolic disorders. In the context of these diseases proteins such as BBS proteins and Rab11 play a role in mediating the effects of dysfunctional RAB8A-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-s111-antibody-mjf-r27-30-bsa-and-azide-free-ab267493--western-blot-img5261.jpg "Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)")
Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.
The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.
Exposure time: 3min
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Recombinant Human STAT1 protein (Tagged) ab276492).
All lanes: Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (Anti-RAB8A (phospho S111) antibody [MJF-R27-30] ab267492) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO at 20 µg
Lane 2: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP at 20 µg
Lane 3: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO at 20 µg
Lane 4: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa
![Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-s111-antibody-mjf-r27-30-bsa-and-azide-free-ab267493--western-blot-img402591.jpg "Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)")
Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)
10 ug/well NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate.
Anti-RAB8A (phospho S111) antibody (Anti-RAB8A (phospho S111) antibody [MJF-R27-30] ab267492) and Anti-RAB8A antibody (Anti-RAB8A antibody [EPR14873] ab188574) at 1/1000 dilution.
Gel: 4-20% TGX gel
Blocking buffer: 5% BSA blocking buffer
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Recombinant Human STAT1 protein (Tagged) ab276492).
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