Rabbit Recombinant Monoclonal RAB8A phospho T72 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Human, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (AB231706), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-t72-antibody-mjf-r20-bsa-and-azide-free-ab231706--western-blot-img152441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (AB231706)")
![Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (AB231706), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-t72-antibody-mjf-r20-bsa-and-azide-free-ab231706--dot-blot-img24712.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (AB231706)")
Publications
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Conformation and dynamics of the kinase domain drive subcellular location and activation of LRRK2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSven H Schmidt,Jui-Hung Weng,Phillip C Aoto,Daniela Boassa,Sebastian Mathea,Steve Silletti,Junru Hu,Maximilian Wallbott,Elizabeth A Komives,Stefan Knapp,Friedrich W Herberg,Susan S TaylorPubMed 34088839
- Structure (London, England : 1993) 28:406-417.e62020Structural Basis for Rab8a Recruitment of RILPL2 via LRRK2 Phosphorylation of Switch 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDieter Waschbüsch et. al.PubMed 32017888
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Expected |
| Synthetic peptide | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 products for Alternative Version
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). May be involved in ciliogenesis (PubMed:21844891, PubMed:30398148). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069).
Alternative names
Ras-related protein Rab-8A, Oncogene c-mel, RAB8, MEL, RAB8A
Recommended products
Rabbit Recombinant Monoclonal RAB8A phospho T72 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Human, Rat samples. Cited in 3 publications.
Alternative names
Ras-related protein Rab-8A, Oncogene c-mel, RAB8, MEL, RAB8A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
MJF-R20
- Purification technique
Affinity purification Protein A
- Specificity
ab230260 cross-reacts with phosphorylated Rab3A, Rab10, Rab35 and Rab43. Please note that the immunogen sequence was derived from Rab8A but is identical to that of Rab8B.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab231706 is the carrier-free version of Anti-RAB8A (phospho T72) antibody [MJF-R20] ab230260.
Please see PMID: 29127256 . Lis P et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
RAB8A is a small GTPase also referred to as Ras-related protein Rab-8A involved in intracellular membrane trafficking. It plays a critical role in the regulation of exocytosis the process by which cells release substances to the extracellular space. RAB8A mainly functions by cycling between an inactive GDP-bound form and an active GTP-bound form facilitating vesicle budding transport and fusion with target membranes. This protein is expressed in various tissues including the brain and pancreas where it assists in important cellular functions. RAB8A has a molecular mass of approximately 24 kDa.
- Biological function summary
The small GTPase RAB8A contributes to the biosynthetic transport from the trans-Golgi network to the plasma membrane. It performs this activity in coordination with other proteins as part of a larger complex. RAB8A also plays an essential role in cilia formation and maintenance affecting cell polarity and signaling functions. The protein's ability to regulate vesicle movement makes it important for cellular homeostasis and communication.
- Pathways
The regulatory function of RAB8A is significant in the ciliary membrane trafficking pathway and the insulin signaling pathway. Within these pathways RAB8A interacts closely with other proteins like Rab11 and Rabin8 which help support its roles in directing vesicle transport. These interactions are essential for proper cellular signaling and the maintenance of polarized cellular structures further supporting the function of various cellular pathways.
- Associated diseases and disorders
The dysfunction of RAB8A has associations with certain ciliopathies notably Bardet-Biedl syndrome. This condition involves abnormalities in cilia structure and function leading to symptoms like kidney disease vision impairment and obesity. Moreover disruptions in RAB8A have connections with insulin resistance implicating it in metabolic disorders. In the context of these diseases proteins such as BBS proteins and Rab11 play a role in mediating the effects of dysfunctional RAB8A-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-t72-antibody-mjf-r20-bsa-and-azide-free-ab231706--western-blot-img152441.jpg "Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)")
Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates Rab8 phosphorylation in MEF (mouse embryonic fibroblasts).
The results show that this antibody recognizes other LRRK2-phosphorylated Rab proteins (Rab3A, Rab10, and Rab35). The phospho-threonine site in Rab8 (T72) differs from that of Rab3A (T86); Rab10 (T73); and Rab43 (T82).
The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB8A (phospho T72) antibody [MJF-R20] ab230260).
All lanes: Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706) at 1/1000 dilution
Lane 1: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 2: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 3: HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab10 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 4: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 5: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab43 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
SecondaryAll lanes: IRDye 800CW secondary antibody at 1/25000 dilution
Predicted band size: 24 kDa
![Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706), expandable thumbnail](https://content.abcam.com/products/images/rab8a-phospho-t72-antibody-mjf-r20-bsa-and-azide-free-ab231706--dot-blot-img24712.jpg "Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)")
Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
Dot Blot - Anti-Rab8A (phospho T72) antibody [MJF-R20] (Anti-RAB8A (phospho T72) antibody [MJF-R20] ab230260) used at a 1/1000 dilution.
Lane 1: Rab8 (phospho T72) peptide.
Lane 2: Rab8 non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) ab97051 secondary antibody used at a 1/100,000 dilution.
Exposure time: 32 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAB8A (phospho T72) antibody [MJF-R20] ab230260).
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