AB235538

Anti-Rab9 antibody - Late Endosome Marker

Name: Anti-Rab9 antibody - Late Endosome Marker (ab235538) | Abcam
Brand: Abcam Limited
SKU: AB235538
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Rab9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAB9A aa 100 to C-terminus.

View Alternative Names

RAB9, RAB9A, Ras-related protein Rab-9A

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

Paraffin-embedded human adrenal gland tissue stained for Rab9 with ab235538 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

Paraffin-embedded human prostate cancer tissue stained for Rab9 with ab235538 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

All lanes:

Western blot - Anti-Rab9 antibody - Late Endosome Marker (ab235538) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RAB9A aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P51151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab9 also known as Ras-related protein Rab-9A is a small GTPase and part of the Rab family with a mass of around 23 kDa. This protein mainly resides in the trans-Golgi network and late endosomes. Rab9 plays an important role in the transport of mannose 6-phosphate receptors between late endosomes and the Golgi apparatus facilitating cargo sorting and vesicular trafficking in cells.

Biological function summary

Rab9 coordinates several intracellular trafficking processes aiding in the regulation of membrane identity. It associates with other effectors such as tail-interacting protein (TIP47) to form complexes on the endosomal surface. These complexes help in vesicular transport ensuring efficient recycling of receptors. Rab9 impacts endosome maturation and transport making it an essential endosome marker.

Pathways

Rab9 influences and is involved in the regulation of the endocytic and secretory pathways. It interacts with proteins like Rab7 another GTPase to manage late endosome and lysosome transport. These interactions underline Rab9's functionalities in intracellular trafficking and recycling processes relying on cooperative interactions with other Rabs and their effectors.

Rab9 plays a role in conditions like Niemann-Pick disease type C and some neurological disorders. Disruption in Rab9 function may affect lipid trafficking and lead to accumulation of cholesterol in late endosomes. This links Rab9 indirectly to proteins such as NPC1 which is known to be implicated in cholesterol homeostasis. Understanding Rab9's involvement can help inform therapeutic strategies targeting these pathways in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB9A is involved in the transport of proteins between the endosomes and the trans-Golgi network (TGN) (PubMed : 34793709). Specifically uses NDE1/NDEL1 as an effector to interact with the dynein motor complex in order to control retrograde trafficking of RAB9-associated late endosomes to the TGN (PubMed : 34793709). Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).

See full target information RAB9A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:1065 PubMed37932706

2023

A pan-cancer analysis revealing the role of LFNG, MFNG and RFNG in tumor prognosis and microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Gong,Chenglong Zheng,Haiying Jia,Yangruiyu Liu,Rui Yang,Zizhou Chen,Yihang Pan,Xiaowu Li,Yuchen Liu

Open life sciences 16:482-494 PubMed34056112

2021

Circ_0013359 facilitates the tumorigenicity of melanoma by regulating miR-136-5p/RAB9A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Yingfa Feng,Jiangang Feng,Jinming Zhang,Lili Huang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Rab9 antibody - Late Endosome Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

Western blot - Anti-Rab9 antibody - Late Endosome Marker (AB235538)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise