Anti-Rabbit IgG antibody [RM1060] - Isotype Control

17 product images

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  ChIP-sequencing - Anti-Rabbit IgG antibody [RM1060] - Isotype Control (ab313801)

  Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^{7 cells and 4 µg of ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.}

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  ChIP-sequencing - Anti-Rabbit IgG antibody [RM1060] - Isotype Control (ab313801)

  Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^{7 cells and 4 µg of ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.}

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  ChIP-sequencing - Anti-Rabbit IgG antibody [RM1060] - Isotype Control (ab313801)

  Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^{7 cells and 4 µg of ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.}

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  Flow Cytometry (Intracellular) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
  Confocal image showing negative staining in C6 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
  Confocal image showing negative staining in NIH/3T3 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
  Confocal image showing negative staining in Hela cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on rat colon.The section was incubated with ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on mouse colon.The section was incubated with ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on human colon.The section was incubated with ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2: ab313801 IP in C6 (rat glial tumor glial cell) whole cell lysate
  Lane 3: Rabbit IgG isotype control (ab313801) instead of Anti-ADK antibody [EPR27497-67] ab307357 in C6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 10 seconds
  The IP experiment was performed by Anti-ADK antibody [EPR27497-67] ab307357 using C6 cells. ab313801 was used as the isotype control instead of Anti-ADK antibody [EPR27497-67] ab307357 in Lane 3.

  All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801) at 1/30 dilution

  All lanes: C6 (rat glial tumor glial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 10s

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  Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 2: ab313801 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 3: Rabbit IgG isotype control (ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in NIH/3T3 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 10 seconds
  The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using NIH/3T3 cells. ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.

  All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801) at 1/30 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 10s

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  Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801)

  Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg U-937 (human histiocytic lymphoma monocyte) whole cell lysate with ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: U-937 (human histiocytic lymphoma monocyte) whole cell lysate
  Lane 2: ab313801 IP in U-937 (human histiocytic lymphoma monocyte) whole cell lysate
  Lane 3: Rabbit IgG isotype control (ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in U937 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 10 seconds
  The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using U937 cells. ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.

  All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (ab313801) at 1/30 dilution

  All lanes: U-937 (human histiocytic lymphoma monocyte) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 10s

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  Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (ab313801)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: 180 seconds

  All lanes: Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (ab313801) at 1/1000 dilution

  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

  Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 180s

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  Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (ab313801)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: 59 seconds

  All lanes: Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (ab313801) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

  Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 59s