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AB190495

Anti-Rabbit IgG Fab region antibody [RMG01]

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(6 Publications)

Goat Recombinant Monoclonal Rabbit IgG Fab region antibody. Suitable for WB, ELISA and reacts with Rabbit samples. Cited in 6 publications.
3 Images
ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)
  • ELISA

Supplier Data

ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)

ELISA analysis of different species' IgG shows ab190495 reacted to the Fab region of Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG as the secondary antibody.

ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)
  • ELISA

Supplier Data

ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)

Titer ELISA analysis of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG was used as the secondary antibody.

Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)
  • WB

Supplier Data

Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (AB190495)

All lanes:

Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495) at 0.2 µg/mL

Lane 1:

Non-reduced Rabbit IgG at 0.02 µg

Lane 2:

Reduced Rabbit IgG at 0.02 µg

Predicted band size: 150 kDa

false

Key facts

Host species

Goat

Clonality

Monoclonal

Clone number

RMG01

Isotype

IgG

Carrier free

No

Reacts with

Rabbit

Applications

ELISA, WB

applications

Specificity

ab190495 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "0.1-0.5 µg/mL", "ELISA-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "0.1-0.5 µg/mL", "ELISA-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "0.1-0.5 µg/mL", "ELISA-species-notes": "<p></p>" }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.01-0.5 µg/mL", "ELISA-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab190495 purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1524714 PubMed40231267

2025

Identification of MAD2L1 as a novel biomarker for hepatoblastoma through bioinformatics and machine learning approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Ying He,Xiwei Hao,Bin Hu,Nan Xia,Chaojin Wang,Xin Chen,Huanyu Zhang,Yuhe Duan,Qinglong Ying,Qian Dong

Cell cycle (Georgetown, Tex.) 21:2179-2191 PubMed35726575

2022

Substance P promotes the progression of bronchial asthma through activating the PI3K/AKT/NF-κB pathway mediated cellular inflammation and pyroptotic cell death in bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Li,Xiao Zhong,Wen-Ting Xu

Journal of gastrointestinal oncology 12:2093-2108 PubMed34790377

2021

Long non-coding RNA regulates the proliferation, migration, and apoptosis of esophageal cancer cells via the -miR-206- axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Gai,Yeqing Huang,Lingling Zhao,Feng Li,Zhixiang Zhuang

Oncology letters 22:584 PubMed34122635

2021

Effect of BRAF-mediated PI3K/Akt/mTOR pathway on biological characteristics and chemosensitivity of NSCLC A549/DDP cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bingnan Ren,Hongtao Liu,Yupeng Yang,Yufei Lian

Experimental and therapeutic medicine 12:4067-4071 PubMed28105136

2016

ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Changshuai Lyu,Yinglan Zhang,Xingnan Zhou,Jinghe Lang

Molecular cancer 14:26 PubMed25644173

2015

Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes.

Applications

ChIP

Species

Unspecified reactive species

Wei Li,Zhiwu Jiang,Tianzhong Li,Xinru Wei,Yi Zheng,Donghai Wu,Lijian Yang,Shaohua Chen,Bing Xu,Mei Zhong,Jue Jiang,Yufeng Hu,Hexiu Su,Minjie Zhang,Xiaojun Huang,Suxia Geng,Jianyu Weng,Xin Du,Pentao Liu,Yangqiu Li,Hudan Liu,Yao Yao,Peng Li
View all publications

Product promise

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