Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free

17 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Vimentin RabMAb (Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547, left panel) (brown) and Rabbit mAb IgG control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, right panel).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Overlay histogram showing A549 (human lung carcinoma) cells stained with Anti-Integrin alpha 2 antibody [EPR5788] ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with Anti-Integrin alpha 2 antibody [EPR5788] ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
  

  Conjugated versions are available for this clone: Alexa Fluor^® 488 (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091), Alexa Fluor^® 647 (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093), R-PE (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478), APC (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Overlay histogram showing K562 (human chronic myelogenous leukemia) cells stained with ab196018 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab196018 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Overlay histogram showing SH-SY5Y (human neuroblastoma) cells stained with Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Overlay histogram showing A549 (human lung carcinoma) cells stained with Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] ab185633 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] ab185633 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Vimentin RabMAb (Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547, left panel) (brown) and Rabbit mAb IgG control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, right panel).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with unpurified Rabbit IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 at 1/10. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with purified Rabbit IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunofluorescent staining of HeLa cells using anti-AIF RabMAb (Anti-AIF antibody [E20] - Mitochondrial Marker ab32516, left panel) (green) and Rabbit mAb IgG control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, right panel). DAPI nuclear staining (blue).
  

  Conjugated versions are available for this clone: Alexa Fluor^® 488 (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091), Alexa Fluor^® 647 (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093), R-PE (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478), APC (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunocytochemistry/immunofluorescence analysis of HeLa cells with purified Rabbit IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Immunocytochemistry/immunofluorescence analysis of HeLa cells with unpurified Rabbit IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  ChIC/CUT&RUN sequencing - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 0.5 µg, 1 µg or 2µg of Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 [EPR25A]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. H3K4me3 (Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade ab213224) used for comparison.
  
  Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
  
  The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
  
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

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  Functional Studies - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-4 (Recombinant human IL-4 protein (Active) ab269147) and anti-IL-4 antibody (Anti-IL-4 antibody [EPR19760-17R] - BSA and Azide free, low endotoxin ab323477) or isotype IgG (ab210849).

  Cell proliferation (%) (A) confirms that the anti-IL-4 antibody (Anti-IL-4 antibody [EPR19760-17R] - BSA and Azide free, low endotoxin ab323477) neutralizes IL-4-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 0.0164 ug/ml (B).

  TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.

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  Functional Studies - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (Recombinant human IL-6 protein (Active) ab259381) and anti-IL-6 antibody (Anti-IL-6- antibody [EPRNGS_IL-6-20] BSA and Azide free, low endotoxin ab323372) or isotype IgG (ab210849).​

  Cell proliferation (%) (A) confirms that the anti-IL-6 antibody (Anti-IL-6- antibody [EPRNGS_IL-6-20] BSA and Azide free, low endotoxin ab323372) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 4.990 ng/ml (B). ​

  TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.

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  Functional Studies - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (Recombinant human IL-6 protein (Active) ab259381) and anti-IL-6 antibody (Anti-IL-6 antibody [EPRNGS_IL-6-10] - BSA and Azide free, low endotoxin ab322419) or isotype IgG (ab210849)
  Cell proliferation (%) (A) confirms that the anti-IL-6 antibody (Anti-IL-6 antibody [EPRNGS_IL-6-10] - BSA and Azide free, low endotoxin ab322419) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 1.450 μg/ml (B). ​
  TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Luminescence was recorded using a Microplate Reader at 560nm emission. ​

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  Western blot - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).

  Negative Control.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST

  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

  All lanes: Western blot - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

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  Functional Studies - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

  Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (Recombinant human IL-6 protein (Active) ab259381) and anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) or isotype IgG (ab210849).

  Cell proliferation (%) (A) confirms that the anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 0.1914 ug/ml (B).

  TF-1 cells were plated at a density of 5 × 10⁴ cells per well in a 96-well plate. A dilution series of the anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) was added to the cells and pre-incubated for 2 hours before IL-6 (Recombinant human IL-6 protein (Active) ab259381) stimulation, and cells were cultured in medium with 5% FBS for a duration of 48 hours. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.